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Title: Differences in the Elemental Isotope Definition May Lead to Errors in Modern Mass-Spectrometry-Based Proteomics
Authors: Claesen, Jürgen
Lermyte, Frederik
Sobott, Frank
Burzykowski, Tomasz
Valkenborg, Dirk
Issue Date: 2015
Citation: ANALYTICAL CHEMISTRY, 87 (21), 10747-10754
Abstract: The elemental isotope definition used to calculate the theoretical masses and isotope distribution of (bio)molecules is considered to be a fixed, universal standard in mass-spectrometrybased proteomics. However, this is an incorrect assumption. In view of the ongoing advances in mass spectrometry technology, and in particular the ever-increasing mass precision, the elemental isotope definition and its variations should be taken into account. We illustrate the effect of the elemental isotope uncertainty on the theoretical and experimental masses with theoretical calculations and examples.
Notes: Claesen, J (reprint author), Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, B-3590 Diepenbeek, Belgium. jurgen.claesen@uhasselt.be
URI: http://hdl.handle.net/1942/19696
DOI: 10.1021/acs.analchem.5b01165
ISI #: 000364354900017
ISSN: 0003-2700
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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