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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/19161

Title: Characterization of the cultivable bacterial populations associated with field grown Brassica napusL.: an evaluation of sampling and isolation protocols
Authors: Croes, Sarah
Weyens, Nele
Colpaert, Jan V.
Vangronsveld, Jaco
Issue Date: 2015
Citation: ENVIRONMENTAL MICROBIOLOGY, 17 (7), p. 2379-2392
Abstract: Plant-associated bacteria are intensively investigated concerning their characteristics for plant growth promotion, biocontrol mechanisms and enhanced phytoremediation efficiency. To obtain endophytes, different sampling and isolation protocols are used although their representativeness is not always clearly demonstrated. The objective of this study was to acquire representative pictures of the cultivable bacterial root, stem and leaf communities for all Brassica napusL. individuals growing on the same field. For each plant organ, genotypic identifications of the endophytic communities were performed using three replicates. Root replicates were composed of three total root systems, whereas stem and leaf replicates needed to consist of six independent plant parts in order to be representative. Greater variations between replicates were found when considering phenotypic characteristics. Correspondence analysis revealed reliable phenotypic results for roots and even shoots, but less reliable ones for leaves. Additionally, realistic Shannon-Wiener biodiversity indices were calculated for all three organs and showed similar Evenness factors. Furthermore, it was striking that all replicates and thus the whole plant contained Pseudomonas and Bacillus strains although aboveground and belowground plant tissues differed in most dominant bacterial genera and characteristics.
Notes: [Croes, Sarah; Weyens, Nele; Colpaert, Jan; Vangronsveld, Jaco] Hasselt Univ, Environm Biol Grp, Ctr Environm Sci, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/19161
DOI: 10.1111/1462-2920.12701
ISI #: 000358114300017
ISSN: 1462-2912
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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