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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/19159

Title: Induction of Oxidative Stress and Antioxidative Mechanisms in Arabidopsis thaliana after Uranium Exposure at pH 7.5
Authors: SAENEN, Eline
HOREMANS, Nele
VANHOUDT, Nathalie
Vandenhove, Hildegarde
BIERMANS, Geert
Van Hees, May
Wannijn, Jean
VANGRONSVELD, Jaco
CUYPERS, Ann
Issue Date: 2015
Publisher: MDPI AG
Citation: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 16 (6), p. 12405-12423
Abstract: To evaluate the environmental impact of uranium (U) contamination, it is important to investigate the effects of U at ecologically relevant conditions. Since U speciation, and hence its toxicity, strongly depends on environmental pH, the present study aimed to investigate dose-dependent effects of U at pH 7.5. Arabidopsis thaliana plants (Mouse-ear Cress) were exposed for three days to different U concentrations at pH 7.5. In the roots, the increased capacities of ascorbate peroxidase and glutathione reductase indicate an important role for the ascorbate-glutathione cycle during U-induced stress. However, a significant decrease in the ascorbate redox state was observed after exposure to 75 and 100 mu M U, indicating that those roots are severely stressed. In accordance with the roots, the ascorbate-glutathione cycle plays an important role in the antioxidative defence systems in A. thaliana leaves exposed to U at pH 7.5 as the ascorbate and glutathione biosynthesis were upregulated. In addition, small inductions of enzymes of the antioxidative defence system were observed at lower U concentrations to counteract the U-induced stress. However, at higher U concentrations it seems that the antioxidative defence system of the leaves collapses as reductions in enzyme activities and gene expression levels were observed.
Notes: [Saenen, Eline; Horemans, Nele; Vanhoudt, Nathalie; Vandenhove, Hildegarde; Biermans, Geert; Van Hees, May; Wannijn, Jean] Belgian Nucl Res Ctr SCK CEN, Biosphere Impact Studies, B-2400 Mol, Belgium. [Saenen, Eline; Biermans, Geert; Vangronsveld, Jaco; Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/19159
DOI: 10.3390/ijms160612405
ISI #: 000357492800035
ISSN: 1422-0067
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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