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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/19012

Title: Label-free Protein Detection Based on the Heat-Transfer Method-A Case Study with the Peanut Allergen Ara h 1 and Aptamer-Based Synthetic Receptors
Authors: PEETERS, Marloes
CLEIJ, Thomas
Jimenez-Monroy, Kathia Lorena
Cornelis, Peter
Perez-Ruiz, Elena
Lammertyn, Jeroen
WAGNER, Patrick
Issue Date: 2015
Citation: ACS APPLIED MATERIALS & INTERFACES, 7 (19), p. 10316-10323
Abstract: Aptamers are an emerging class of molecules that, because of the development of the systematic 'evolution of ligands by exponential enrichment (SELEX) proceSs, can recognize Virtually every target ranging from ions, to proteins, and even whole Cells. Although there are niany techniques capable of detecting template molectjles with aptamer-based systems with high specificity and selectivity, they lack the possibility of integrating them into a compact and portable biosensor setup. Therefore, we Will present the heat-transfer method (HTM) as an interesting alternative because this offers detection in a fast and low-cost manner and has the possibility of performing experiments with a fully integrated device. This concept has been demonstrated for a variety of applications including DNA mutation analysis and screening, Of cancer cells, To the best our knowledge, this is the first report on HTM-based detection of proteins, in this case specifically with aptamer-type receptors. For proof-of-principle purposes, measurements will be performed with the peanut allergen Ara h 1 and results indicate detection limits in the lower nanornolar regime in buffer liquid. As a first proof-of-application, spiked Ara h 1 solutions will be studied in a food matrix of dissolved peanut butter. Reference experiments with the quartz-crystal microbalance will allow for an estimate of the areal density of aptamer molecules on the sensor-chip surface.
Notes: [Peeters, Marloes; Jimenez-Monroy, Kathia Lorena; Wackers, Gideon; Thoelen, Ronald; De Ceuninck, Ward; Wagner, Patrick] Hasselt Univ, Inst Mat Res, B-3590 Diepenbeek, Belgium. [Peeters, Marloes] Queen Mary Univ London, Sch Biol & Chem Sci, London E1 4NS, England. [van Grinsven, Bart; Cleij, Thomas J.] Maastricht Univ, Maastricht Sci Programme, NL-6200 MD Maastricht, Netherlands. [Cornelis, Peter; Wackers, Gideon; Wagner, Patrick] Katholieke Univ Leuven, Soft Matter & Biophys Sect, Dept Phys & Astron, B-3001 Leuven, Belgium. [Thoelen, Ronald; De Ceuninck, Ward] IMEC Vzw, Div IMOMEC, B-3590 Diepenbeek, Belgium. [Perez-Ruiz, Elena; Lammertyn, Jeroen] Univ Leuven, KU Leuven, BIOSYST MeBioS, B-3000 Leuven, Belgium.
URI: http://hdl.handle.net/1942/19012
DOI: 10.1021/acsami.5b00994
ISI #: 000355055000028
ISSN: 1944-8244
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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