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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/18993

Title: An effective plasma membrane proteomics approach for small tissue samples
Authors: Smolders, Katrien
Lombaert, Nathalie
VALKENBORG, Dirk
Baggerman, Geert
Arckens, Lutgarde
Issue Date: 2015
Publisher: NATURE PUBLISHING GROUP
Citation: SCIENTIFIC REPORTS, 5
Abstract: Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of >1,600 proteins of which >60% are associated with the plasma membrane, including (G-protein coupled) receptors, ion channels and transporters, and this from mm(3)-scale tissue.
Notes: [Smolders, Katrien; Lombaert, Nathalie; Arckens, Lutgarde] Katholieke Univ Leuven, Dept Biol, Lab Neuroplast & Neuroprote, Leuven, Belgium. [Valkenborg, Dirk; Baggerman, Geert] Vlaamse Instelling Technol Onderzoek, Unit Environm Risk & Hlth, B-2400 Mol, Belgium. [Valkenborg, Dirk; Baggerman, Geert] UAntwerp, Ctr Prote, Antwerp, Belgium. [Valkenborg, Dirk] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Hasselt, Belgium.
URI: http://hdl.handle.net/1942/18993
DOI: 10.1038/srep10917
ISI #: 000355865400001
ISSN: 2045-2322
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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