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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/18901

Title: Effect of Cholesterol on the Phase Behavior of Solid-Supported Lipid Vesicle Layers
Authors: LOSADA-PEREZ, Patricia
Yongabi, D.
WAGNER, Patrick
Issue Date: 2015
Citation: JOURNAL OF PHYSICAL CHEMISTRY B, 119 (15), p. 4985-4992
Abstract: The interest in solid-supported biomimetic membranes stems from their utility in nanotechnology and biosensing. In particular, supported lipid vesicles (SLVs) have become popular in both fundamental biophysical studies and pharmaceutical screening applications. It is thus essential to gain information on the structural properties and phase behavior of SLVs. Here we report on a study on the influence of cholesterol on the phase behavior of SLVs of saturated phospholipids by using quartz crystal microbalance with dissipation monitoring, a label-free and nonintrusive surface-sensitive technique. Two complementary approaches have been used, a Voigt-based viscoelastic model yielding shear viscosity temperature profiles and the first-order derivative of the frequency (mass-sensitive) shifts. Anomalies in the shear viscosity and extrema in the first-order derivative frequency curves stand as a token of the main phase transition and provide information on its gradual suppression upon addition of cholesterol. This method proves convenient for its small sample volume needed, its short temperature equilibration time and the non-necessity of external labels. This work can be regarded as a starting point for further studies on more rare lipid systems and different geometries, such as tethered SLVs or biologically relevant vesicles produced by living cells.
Notes: [Losada-Perez, P.; Khorshid, M.; Yongabi, D.; Wagner, P.] Hasselt Univ, IMO, Inst Mat Res, B-3590 Diepenbeek, Belgium. [Losada-Perez, P.] IMEC VZW, Div IMOMEC, B-3590 Diepenbeek, Belgium. [Khorshid, M.; Wagner, P.] Katholieke Univ Leuven, Dept Phys & Astron, Soft Matter & Biophys Sect, B-3001 Louvain, Belgium.
URI: http://hdl.handle.net/1942/18901
DOI: 10.1021/acs.jpcb.5b00712
ISI #: 000353249400009
ISSN: 1520-6106
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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