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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/18719

Title: Distinct In Vitro Properties of Embryonic and Extraembryonic Fibroblast-Like Cells Are Reflected in Their In Vivo Behavior Following Grafting in the Adult Mouse Brain
Authors: Costa, Roberta
Bergwerf, Irene
De Vocht, Nathalie
Praet, Jelle
Daans, Jasmijn
Le Blon, Debbie
Hoomaert, Chloe
Reekmans, Kristien
HENS, Niel
Goossens, Herman
Berneman, Zwi
Parolini, Ornella
Alviano, Francesco
Ponsaerts, Peter
Issue Date: 2015
Citation: CELL TRANSPLANTATION, 24 (2), p. 223-233
Abstract: Although intracerebral transplantation of various fibroblast(-like) cell populations has been shown feasible, little is known about the actual in vivo remodeling of these cellular grafts and their environment. In this study, we aimed to compare the in vitro and in vivo behavior of two phenotypically similar but developmentally distinct fibroblast-like cell populations, namely, mouse embryonic fibroblasts (mEFs) and mouse fetal membrane-derived stromal cells (mFMSCs). While both mEFs and mFMSCs are readily able to reduce TNF-alpha secretion by LPS/IFN-gamma-activated BV-2 microglia, mFMSCs and mEFs display strikingly opposite behavior with regard to VEGF production under normal and inflammatory conditions. Whereas mFMSCs downregulate VEGF production upon coculture with LPS/IFN-gamma-activated BV-2 microglia, mEFs upregulate VEGF production in the presence of LPS/IFN-gamma-activated BV-2 microglia. Subsequently, in vivo grafting of mFMSCs and IDEFs revealed no difference in microglial and astroglial responses toward the cellular grafts. However, mFMSC grafts displayed a lower degree of neoangiogenesis compared to mEF grafts, thereby potentially explaining the lower cell number able to survive in mFMSC grafts. In summary, our results suggest that physiological differences between fibroblast-like cell populations might lie at the basis of variations in histopathological and/or clinical outcome following cell grafting in mouse brain.
Notes: Ponsaerts, P (reprint author),Univ Antwerp, Vaccine & Infect Dis Inst Vaxinfectio, Lab Expt Hematol, Expt Cell Transplantat Grp, Campus Drie Eiken CDE-56-51,Univ Pl 1, B-2610 Antwerp, Wilrijk, Belgium. peter.ponsaerts@uantwerpen.be
URI: http://hdl.handle.net/1942/18719
DOI: 10.3727/096368913X676196
ISI #: 000351251400008
ISSN: 0963-6897
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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