Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/18712

Title: Imaging the Zebrafish Dentition: From Traditional Approaches to Emerging Technologies
Authors: Bruneel, Bart
Mathae, Markus
Weninger, Wolfgang J.
Huysseune, Ann
Issue Date: 2015
Citation: ZEBRAFISH, 12 (1), p. 1-10
Abstract: The zebrafish, a model organism for which a plethora of molecular and genetic techniques exists, has a lifelong replacing dentition of 22 pharyngeal teeth. This is in contrast to the mouse, which is the key organism in dental research but whose teeth are never replaced. Employing the zebrafish as the main organism to elucidate the mechanisms of continuous tooth replacement, however, poses at least one major problem, related to the fact that all teeth are located deep inside the body. Investigating tooth replacement thus relies on conventional histological methods, which are often laborious, time-consuming and can cause tissue deformations. In this review, we investigate the advantages and limitations of adapting current visualization techniques to dental research in zebrafish. We discuss techniques for fast sectioning, such as vibratome sectioning and high-resolution episcopic microscopy, and methods for in toto visualization, such as Alizarin red staining, micro-computed tomography, and optical projection tomography. Techniques for in vivo imaging, such as two-photon excitation fluorescence and second harmonic generation microscopy, are also covered. Finally, the possibilities of light sheet microscopy are addressed.
Notes: Bruneel, B (reprint author), Univ Ghent, Proeftuinstr 86 N1, B-9000 Ghent, Belgium. bart.bruneel@ugent.be
URI: http://hdl.handle.net/1942/18712
DOI: 10.1089/zeb.2014.0980
ISI #: 000348323600001
ISSN: 1545-8547
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

Files in This Item:

There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.