Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/18699

Title: Clinical-scale in vitro expansion preserves biological characteristics of cardiac atrial appendage stem cells
Authors: WINDMOLDERS, Severina
FANTON, Yanick
Rummens, Jean-Luc
Issue Date: 2015
Citation: CELL PROLIFERATION, 48 (2), p. 175-186
Abstract: Objectives: Cardiac atrial appendage stem cells (CASCs) have recently emerged as an attractive candidate for cardiac regeneration after myocardial infarction. As with other cardiac stem cells, CASCs have to be expanded ex vivo to obtain clinically relevant cell numbers. However, foetal calf serum (FCS), which is routinely used for cell culturing, is unsuitable for clinical purposes, and influence of long-term in vitro culture on CASC behaviour is unknown. Materials and methodsWe examined effects on CASC biology of prolonged expansion, and evaluated a culture protocol suitable for human use. Results: In FCS-supplemented medium, CASCs could be kept in culture for 55.753.63days, before reaching senescence. Despite a small reduction in numbers of proliferating CASCs (1.37 +/- 0.52% per passage) and signs of progressive telomere shortening (0.04 +/- 0.02kb per passage), their immunophenotype and myocardial differentiation potential remained unaffected during the entire culture period. The cells were successfully expanded in human platelet plasma supernatant, while maintaining their biological properties. Conclusions: We successfully developed a protocol for long-term culture, to obtain clinically relevant CASC numbers, while retaining their cardiogenic potential. These insights in CASC biology and optimization of a humanized platelet-based culture method are an important step towards clinical application of CASCs for cardiac regenerative medicine.
Notes: Correspondence: K. Hensen, Laboratory of Experimental Hematology, Jessa Hospital, Campus Virga Jesse, Stadsomvaart 11, 3500 Hasselt, Belgium. Tel.: (+32) (0)11338266; Fax: (+32) (0)11338208; karen.hensen@jessazh.be
URI: http://hdl.handle.net/1942/18699
DOI: 10.1111/cpr.12166
ISI #: 000350978400006
ISSN: 0960-7722
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

Files in This Item:

Description SizeFormat
published version1.41 MBAdobe PDF

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.