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|Title: ||Heat-Transfer-Method-Based Cell Culture Quality Assay through Cell Detection by Surface Imprinted Polymers|
|Authors: ||EERSELS, Kasper|
VAN GRINSVEN, Bart
Bos, Gerard M. J.
Germeraad, Wilfred T. V.
DE CEUNINCK, Ward
|Issue Date: ||2015|
|Publisher: ||AMER CHEMICAL SOC|
|Citation: ||LANGMUIR, 31 (6), p. 2043-2050|
|Abstract: ||Previous work has indicated that surface imprinted polymers (SIPs) allow for highly specific cell detection through macromolecular cell imprints. The combination of SIPs with a heat-transfer-based read-out technique has led to the development of a selective, label-free, low-cost, and user-friendly cell detection assay. In this study, the breast cancer cell line ZR-75-1 is used to assess the potential of the platform for monitoring the quality of a cell culture in time. For this purpose, we show that the proposed methodology is able to discriminate between the original cell line (adherent growth, ZR-75-1a) and a descendant cell line (suspension growth, ZR-75-1s). Moreover, ZR-75-1a cells were cultured for a prolonged period of time and analyzed using the heat-transfer method (HTM) at regular time intervals. The results of these experiments demonstrate that the thermal resistance (R-th) signal decays after a certain number of cell culture passages. This can likely be attributed to a compromised quality of the cell culture due to cross-contamination with the ZR-75-1s cell line, a finding that was confirmed by classical STR DNA profiling. The cells do not express the same functional groups on their membrane, resulting in a weaker bond between cell and imprint, enabling cell removal by mechanical friction, provided by flushing the measuring chamber with buffer solution. These findings were further confirmed by HTM and illustrate that the biomimetic sensor platform can be used as an assay for monitoring the quality of cell cultures in time.|
|Notes: ||[Eersels, Kasper; Khorshid, Mehran; Thoelen, Ronald; De Ceuninck, Ward; Wagner, Patrick] Hasselt Univ, Inst Mat Res IMO, B-3590 Diepenbeek, Belgium. [van Grinsven, Bart; Dilien, Hanne; Cleij, Thomas J.] Maastricht Univ, Maastricht Sci Programme, NL-6200 MD Maastricht, Netherlands. [Somers, Veerle] Hasselt Univ, Biomed Res Inst, Sch Life Sci, Transnat Univ Limburg, B-3590 Diepenbeek, Belgium. [Puttmann, Christiane; Stein, Christoph; Barth, Stefan] RWTH Aachen Univ Clin, Inst Appl Med Engn, Dept Expt Med & Immunotherapy, D-52074 Aachen, Germany. [Bos, Gerard M. J.; Germeraad, Wilfred T. V.] Maastricht Univ, Med Ctr, Dept Internal Med, Div Haematol, NL-6202 AZ Maastricht, Netherlands. [Thoelen, Ronald; De Ceuninck, Ward; Wagner, Patrick] IMEC VZW, IMOMEC Div, B-3590 Diepenbeek, Belgium.
|ISI #: ||000349805900022|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2016|
|Appears in Collections: ||Research publications|
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|published version||5.77 MB||Adobe PDF|
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