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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/18669

Title: Melittin disruption of raft and non-raft-forming biomimetic membranes: A study by quartz crystal microbalance with dissipation monitoring
Authors: LOSADA-PEREZ, Patricia
KHORSHID, Mehran
HERMANS, Chris
Robijns, T.
PEETERS, Marloes
Jimenez-Monroy, K. L.
Truong, L. T. N.
WAGNER, Patrick
Issue Date: 2014
Publisher: ELSEVIER SCIENCE BV
Citation: COLLOIDS AND SURFACES B-BIOINTERFACES, 123, p. 938-944
Abstract: In this work we examine the role of lateral phase separation in cholesterol-containing biomimetic membranes on the disrupting action of melittin using a label-free surface-sensitive technique, quartz crystal microbalance with dissipation monitoring (QCM-D). Melittin disruption mechanisms depend strongly on the geometry of the lipid layer; however, despite the interplay between layer geometry/thickness and melittin activity, results indicate that the presence of lipid heterogeneity and lateral phase separation greatly influences the disrupting efficiency of melittin. In homogeneous non-raft forming membranes with high cholesterol content, melittin spontaneous activity is strongly delayed compared to heterogeneous raft-forming systems with the same amount of cholesterol. These results confirm the importance of lateral phase separation as a determinant factor in peptide activity. The information provided can be used for the design of more efficient antimicrobial peptides and the possibility of using a label-free approach for tailored-membranes and interactions with other types of peptides, such as amyloid peptides. (C) 2014 Elsevier B.V. All rights reserved.
Notes: [Losada-Perez, P.; Khorshid, M.; Hermans, C.; Robijns, T.; Peeters, M.; Jimenez-Monroy, K. L.; Wagner, P.] Hasselt Univ, IMO, B-3590 Diepenbeek, Belgium. [Losada-Perez, P.; Wagner, P.] IMEC Vzw, Div IMOMEC, B-3590 Diepenbeek, Belgium. [Truong, L. T. N.] Hanoi Univ Sci & Technol 1, Sch Engn Phys, Hanoi, Vietnam.
URI: http://hdl.handle.net/1942/18669
DOI: 10.1016/j.colsurfb.2014.10.048
ISI #: 000347580500118
ISSN: 0927-7765
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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