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|Title: ||Melittin disruption of raft and non-raft-forming biomimetic membranes: A study by quartz crystal microbalance with dissipation monitoring|
|Authors: ||LOSADA-PEREZ, Patricia|
Jimenez-Monroy, K. L.
Truong, L. T. N.
|Issue Date: ||2014|
|Publisher: ||ELSEVIER SCIENCE BV|
|Citation: ||COLLOIDS AND SURFACES B-BIOINTERFACES, 123, p. 938-944|
|Abstract: ||In this work we examine the role of lateral phase separation in cholesterol-containing biomimetic membranes on the disrupting action of melittin using a label-free surface-sensitive technique, quartz crystal microbalance with dissipation monitoring (QCM-D). Melittin disruption mechanisms depend strongly on the geometry of the lipid layer; however, despite the interplay between layer geometry/thickness and melittin activity, results indicate that the presence of lipid heterogeneity and lateral phase separation greatly influences the disrupting efficiency of melittin. In homogeneous non-raft forming membranes with high cholesterol content, melittin spontaneous activity is strongly delayed compared to heterogeneous raft-forming systems with the same amount of cholesterol. These results confirm the importance of lateral phase separation as a determinant factor in peptide activity. The information provided can be used for the design of more efficient antimicrobial peptides and the possibility of using a label-free approach for tailored-membranes and interactions with other types of peptides, such as amyloid peptides. (C) 2014 Elsevier B.V. All rights reserved.|
|Notes: ||[Losada-Perez, P.; Khorshid, M.; Hermans, C.; Robijns, T.; Peeters, M.; Jimenez-Monroy, K. L.; Wagner, P.] Hasselt Univ, IMO, B-3590 Diepenbeek, Belgium. [Losada-Perez, P.; Wagner, P.] IMEC Vzw, Div IMOMEC, B-3590 Diepenbeek, Belgium. [Truong, L. T. N.] Hanoi Univ Sci & Technol 1, Sch Engn Phys, Hanoi, Vietnam.|
|ISI #: ||000347580500118|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2016|
|Appears in Collections: ||Research publications|
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