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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/18661

Title: Impact of diamond nanoparticles on neural cells
Authors: Vaitkuviene, Aida
Ratautaite, Vilma
Ramanaviciene, Almira
SANEN, Kathleen
Petrakova, Vladimira
Kaseta, Vytautas
Ramanauskaite, Giedre
Biziuleviciene, Gene
Ramanavicius, Arunas
Issue Date: 2015
Citation: MOLECULAR AND CELLULAR PROBES, 29 (1), p. 25-30
Abstract: Diamond nanoparticles (DNPs) are very attractive for biomedical applications, particularly for bioimaging. The aim of this study was to evaluate the impact of DNPs on neural cancer cells and thus to assess the possible application of DNPs for these cells imaging. For this purpose, the neuroblastoma SH-SY5Y cell line was chosen. Cells were cultured in medium with different concentrations (15, 50, 100 and 150 mu g/ml) of DNPs. After 48 h of incubation, cell metabolic activity was evaluated by the XTT assay. For assessment of cellular metabolic activity, cells were also cultured on differently terminated nanocrystalline diamond (NCD) coatings in medium with 150 mu g/ml of DNPs. Cell adhesion and morphology were evaluated by brightfield microscopy. Diamond nanoparticle internalization was determined by confocal microscopy. The obtained results showed that low concentrations (15, 50 and 100 mu g/ml) of nanoparticles did not significantly affect the SH-SY5Y cell metabolic activity. However, a higher concentration (150 mu g/ml) of DNPs statistically significantly reduced SH-SY5Y cell metabolic activity. After 48 h incubation with 150 mu g/ml DNPs, cell metabolic activity was 23% lower than in medium without DNPs on standard tissue culture polystyrene. (C) 2014 Elsevier Ltd. All rights reserved.
Notes: [Vaitkuviene, Aida; Ratautaite, Vilma; Ramanavicius, Arunas] Vilnius Univ, Fac Chem, Dept Phys Chem, LT-03225 Vilnius, Lithuania. [Vaitkuviene, Aida; Kaseta, Vytautas; Ramanauskaite, Giedre; Biziuleviciene, Gene] State Res Inst Ctr Innovat Med, Dept Stem Cell Biol, LT-01102 Vilnius, Lithuania. [Ramanaviciene, Almira] Vilnius Univ, Fac Chem, NanoTechnas Ctr Nanotechnol & Mat Sci, LT-03225 Vilnius, Lithuania. [Sanen, Kathleen; Paesen, Rik; Ameloot, Marcel; Nesladek, Milos] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Petrakova, Vladimira] Czech Tech Univ, Fac Biomed Engn, Kladno 27201, Czech Republic. [McDonald, Matthew; Vahidpour, Farnoosh; Nesladek, Milos] Hasselt Univ, Inst Mat Res, Div IMOMEC, B-3590 Diepenbeek, Belgium. [Ramanavicius, Arunas] State Sci Res Inst, Ctr Phys Sci & Technol, Inst Semicond Phys, Lab NanoBioTechnol,Dept Mat Sci & Elect, LT-01108 Vilnius, Lithuania.
URI: http://hdl.handle.net/1942/18661
DOI: 10.1016/j.mcp.2014.10.005
ISI #: 000350532300005
ISSN: 0890-8508
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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