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|Title: ||Histological characterization and quantification of cellular events following neural and fibroblast(-like) stem cell grafting in healty and demyelinated CNS tissue|
|Authors: ||Praet, J.|
de Vocht, N.
Le Blon, D.
Van der Linden, A.
|Issue Date: ||2014|
|Publisher: ||Springer Science + Business Media|
|Citation: ||Christ, Bruno; Oerlecke, Jana; Stock, Peggy (Ed.). Animal models for stem cell therapy, p. 265-283|
|Series/Report: ||Methods in Molecular Biology|
|Series/Report no.: ||1213|
|Abstract: ||Preclinical animal studies involving intracerebral (stem) cell grafting are gaining popularity in many laboratories due to the reported beneficial effects of cell grafting on various diseases or traumata of the central nervous system (CNS). In this chapter, we describe a histological workflow to characterize and quantify cellular events following neural and fibroblast(-like) stem cell grafting in healthy and demyelinated CNS tissue. First, we provide standardized protocols to isolate and culture eGFP+ neural and fibroblast(-like) stem cells from embryonic mouse tissue. Second, we describe flow cytometric procedures to determine cell viability, eGFP transgene expression, and the expression of different stem cell lineage markers. Third, we explain how to induce reproducible demyelination in the CNS of mice by means of cuprizone administration, a validated mouse model for human multiple sclerosis. Fourth, the technical procedures for cell grafting in the CNS are explained in detail. Finally, an optimized and validated workflow for the quantitative histological analysis of cell graft survival and endogenous astroglial and microglial responses is provided.|
|Notes: ||[Praet, Jelle; Reekmans, Kristien; de Vocht, Nathalie; Le Blon, Debbie; Hoornaert, Chloe; Daans, Jasmijn; Berneman, Zwi; Ponsaerts, Peter] Univ Antwerp, Lab Expt Hematol, B-2020 Antwerp, Belgium. [Praet, Jelle; Santermans, Eva; Reekmans, Kristien; de Vocht, Nathalie; Le Blon, Debbie; Hoornaert, Chloe; Daans, Jasmijn; Goossens, Herman; Berneman, Zwi; Hens, Niel; Ponsaerts, Peter] Univ Antwerp, Vaccine & Infect Dis Inst Vaxinfectio, B-2020 Antwerp, Belgium. [Praet, Jelle; de Vocht, Nathalie; Van der Linden, Annemie] Univ Antwerp, Bioimaging Lab, B-2020 Antwerp, Belgium. [Santermans, Eva; Hens, Niel] Hasselt Univ, Ctr Stat, I Biostat, Hasselt, Belgium. [Hens, Niel] Univ Antwerp, Chermid, B-2020 Antwerp, Belgium.|
|ISI #: ||000342900700023|
|Type: ||Book Section|
|Appears in Collections: ||Research publications|
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