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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/18302

Title: Validation of Microtox as a first screening tool for waste classification
Authors: Weltens, R.
Deprez, Karolien
Michiels, Luc
Issue Date: 2014
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Citation: WASTE MANAGEMENT, 34 (12), p. 2427-2433
Abstract: The Waste Framework Directive (WFD; 2008/98/EG) describes how waste materials are to be classified as hazardous or not. For complex waste materials chemical analyses are often not conclusive and the WFD provides the possibility to assess the hazardous properties by testing on the waste materials directly. As a methodology WFD refers to the protocols described in the CLP regulation (regulation on Classification, Labeling and Packaging of chemicals) but the toxicity tests on mammals are not acceptable for waste materials. The DISCRISET project was initiated to investigate the suitability of alternative toxicity tests that are already in use in pharmaceutical applications, for the toxicological hazard assessment of complex waste materials. Results indicated that Microtox was a good candidate as a first screening test in a tiered approached hazard assessment. This is now further validated in the present study. The toxic responses measured in Microtox were compared to biological responses in other bioassays for both organic and inorganic fractions of the wastes. Both fractions contribute to the toxic load of waste samples. Results show that the Microtox test is indeed a good and practical screening tool for the organic fraction. A screening threshold (ST) of 5 geq/l as the ECK, value in Microtox is proposed as this ST allows to recognize highly toxic samples in the screening test. The data presented here show that the Microtox toxicity response at this ST is not only predictive for acute toxicity in other organisms but also for sub lethal toxic effects of the organic fraction. This limit value has to be further validated. For the inorganic fraction no specific biotest can be recommended as a screening test, but the use of direct toxicity assessment is also preferable for this fraction as metal speciation is an important issue to define the toxic load of elutriate fractions. A battery of 3 tests (Microtox, Daphnia and Algae) for direct toxicity assessment of this fraction is recommended in literature, but including tests for mechanistic toxicity might be useful. (C) 2014 Elsevier Ltd. All rights reserved.
Notes: [Weltens, R.] VITO, Flemish Inst Technol Res, B-2400 Mol, Belgium. [Deprez, K.; Michiels, L.] UH Hasselt Univ, BIOMED, B-3500 Hasselt, Belgium.
URI: http://hdl.handle.net/1942/18302
DOI: 10.1016/j.wasman.2014.08.001
ISI #: 000347019700002
ISSN: 0956-053X
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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