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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/18290

Title: Time-resolved fluorescence methods (IUPAC Technical Report)
Authors: Lemmetyinen, Helge
Tkachenko, Nikolai V.
Valeur, Bernard
Hotta, Jun-ichi
AMELOOT, Marcel
Ernsting, Nikolaus P.
Gustavsson, Thomas
Boens, Noel
Issue Date: 2014
Publisher: WALTER DE GRUYTER GMBH
Citation: PURE AND APPLIED CHEMISTRY, 86 (12), p. 1969-1998
Abstract: This IUPAC Technical Report describes and compares the currently applied methods for measuring and analyzing time-resolved fluorescence traces using phase-modulation fluorometry as well as pulse fluorometry (direct emission decay measurements, single-photon timing, streak camera measurements, fluorescence upconversion, and optical Kerr gating). The paper starts with a brief description of the basic principles for time and frequency domain fluorescence spectroscopy. The fundamental equations are given, and recommendations for adequate use are emphasized. The up-to-date, commonly employed excitation sources and photodetectors are described in detail. The analysis of time-resolved fluorescence data is discussed. Attention is paid to possible artifacts, and remedies are presented on how to avoid them or to account for them. Finally, fluorescence lifetime standards for the nanosecond and picosecond timescales are collected.
Notes: [Lemmetyinen, Helge; Tkachenko, Nikolai V.] Tampere Univ Technol, Dept Chem & Bioengn, FI-33101 Tampere, Finland. [Valeur, Bernard] Conservatoire Natl Arts & Metiers, Dept CASER, F-75003 Paris, France. [Valeur, Bernard] Ecole Normale Super, Lab PPSM, F-94235 Cachan, France. [Hotta, Jun-ichi] Yamagata Univ, Grad Sch Sci & Engn, Yonezawa, Yamagata 9928510, Japan. [Ameloot, Marcel] Hasselt Univ, B-3500 Hasselt, Belgium. [Ernsting, Nikolaus P.] Humboldt Univ, Dept Chem, D-12489 Berlin, Germany. [Gustavsson, Thomas] CENS, Lab Leon Brillouin, CNRS, IRAMIS,LIDYL,Lab Francis Perrin,URA 2453, F-91191 Gif Sur Yvette, France. [Boens, Noel] Katholieke Univ Leuven, Dept Chem, B-3001 Leuven, Belgium.
URI: http://hdl.handle.net/1942/18290
DOI: 10.1515/pac-2013-0912
ISI #: 000347554700008
ISSN: 0033-4545
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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