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|Title: ||Time-resolved fluorescence methods (IUPAC Technical Report)|
|Authors: ||Lemmetyinen, Helge|
Tkachenko, Nikolai V.
Ernsting, Nikolaus P.
|Issue Date: ||2014|
|Publisher: ||WALTER DE GRUYTER GMBH|
|Citation: ||PURE AND APPLIED CHEMISTRY, 86 (12), p. 1969-1998|
|Abstract: ||This IUPAC Technical Report describes and compares the currently applied methods for measuring and analyzing time-resolved fluorescence traces using phase-modulation fluorometry as well as pulse fluorometry (direct emission decay measurements, single-photon timing, streak camera measurements, fluorescence upconversion, and optical Kerr gating). The paper starts with a brief description of the basic principles for time and frequency domain fluorescence spectroscopy. The fundamental equations are given, and recommendations for adequate use are emphasized. The up-to-date, commonly employed excitation sources and photodetectors are described in detail. The analysis of time-resolved fluorescence data is discussed. Attention is paid to possible artifacts, and remedies are presented on how to avoid them or to account for them. Finally, fluorescence lifetime standards for the nanosecond and picosecond timescales are collected.|
|Notes: ||[Lemmetyinen, Helge; Tkachenko, Nikolai V.] Tampere Univ Technol, Dept Chem & Bioengn, FI-33101 Tampere, Finland. [Valeur, Bernard] Conservatoire Natl Arts & Metiers, Dept CASER, F-75003 Paris, France. [Valeur, Bernard] Ecole Normale Super, Lab PPSM, F-94235 Cachan, France. [Hotta, Jun-ichi] Yamagata Univ, Grad Sch Sci & Engn, Yonezawa, Yamagata 9928510, Japan. [Ameloot, Marcel] Hasselt Univ, B-3500 Hasselt, Belgium. [Ernsting, Nikolaus P.] Humboldt Univ, Dept Chem, D-12489 Berlin, Germany. [Gustavsson, Thomas] CENS, Lab Leon Brillouin, CNRS, IRAMIS,LIDYL,Lab Francis Perrin,URA 2453, F-91191 Gif Sur Yvette, France. [Boens, Noel] Katholieke Univ Leuven, Dept Chem, B-3001 Leuven, Belgium.|
|ISI #: ||000347554700008|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2016|
|Appears in Collections: ||Research publications|
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