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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/18080

Title: Dynamic range extension of hybridization sensors
Authors: Hadiwikarta, W. W.
Carlon, E.
Issue Date: 2015
Citation: BIOSENSORS & BIOELECTRONICS, 64, p. 411-415
Abstract: In hybridization based nucleic acid sensors the stringency of hybridization poses a challenge to design and experiment. For a given set of experimental parameters the affinity window of probe target interaction is always limited and vice versa for a given probe set design, changes in experimental conditions can easily bring some measurements out of detection range. In this paper we introduce and apply a strategy to extend this dynamic range for affinity sensors, sensors which measure the amount of hybridized molecules after equilibrium is reached. The method relies on concepts of additivity of nucleic acids hybridization free energies and on equilibrium isotherms. It consists in combining the measurements from probes with different lengths, by appropriately rescaling the measured signals. We test the validity of the approach on experiments and show that by combining probes with hybridizing regions of length 21, 23 and 25 nucleotides we manage to extend the dynamic range of the intensity signals by a factor of 25. The presented concept is easy to extend, platform free and applies to any hybridization based affinity sensor. (C) 2014 Elsevier B.V. All rights reserved.
Notes: [Hadiwikarta, W. W.; Hooyberghs, J.] Vlaamse Instelling Technol Onderzoek, Flemish Inst Technol Res, B-2400 Mol, Belgium. [Hadiwikarta, W. W.; Carlon, E.] KULeuven, Inst Theoret Phys, B-3001 Leuven, Belgium. [Hooyberghs, J.] Hasselt Univ, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/18080
DOI: 10.1016/j.bios.2014.09.043
ISI #: 000345059100059
ISSN: 0956-5663
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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