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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/17931

Title: Compositional Changes of B and T Cell Subtypes during Fingolimod Treatment in Multiple Sclerosis Patients: A 12-Month Follow-Up Study
Authors: CLAES, Nele
DHAEZE, Tessa
FRAUSSEN, Judith
BROUX, Bieke
VAN WIJMEERSCH, Bart
STINISSEN, Piet
HELLINGS, Niels
SOMERS, Veerle
Hupperts, Raymond
Issue Date: 2014
Citation: PloS one, 9 (10), p. 1-8
Abstract: Background and objective: The long term effects of fingolimod, an oral treatment for relapsing-remitting (RR) multiple sclerosis (MS), on blood circulating B and T cell subtypes in MS patients are not completely understood. This study describes for the first time the longitudinal effects of fingolimod treatment on B and T cell subtypes. Furthermore, expression of surface molecules involved in antigen presentation and costimulation during fingolimod treatment are assessed in MS patients in a 12 month follow-up study. Methods: Using flow cytometry, B and T cell subtypes, and their expression of antigen presentation, costimulation and migration markers were measured during a 12 month follow-up in the peripheral blood of MS patients. Data of fingolimodtreated MS patients (n = 49) were compared to those from treatment-naive (n = 47) and interferon-treated (n = 27) MS patients. Results: In the B cell population, we observed a decrease in the proportion of non class-switched and class-switched memory B cells (p,0.001), both implicated in MS pathogenesis, while the proportion of naive B cells was increased during fingolimod treatment in the peripheral blood (PB) of MS patients (p,0.05). The remaining T cell population, in contrast, showed elevated proportions of memory conventional and regulatory T cells (p,0.01) and declined proportions of naive conventional and regulatory cells (p,0.05). These naive T cell subtypes are main drivers of MS pathogenesis. B cell expression of CD80 and CD86 and programmed death (PD) -1 expression on circulating follicular helper T cells was increased during fingolimod follow-up (p,0.05) pointing to a potentially compensatory mechanism of the remaining circulating lymphocyte subtypes that could provide additional help during normal immune responses. Conclusions: MS patients treated with fingolimod showed a change in PB lymphocyte subtype proportions and expression of functional molecules on T and B cells, suggesting an association with the therapeutic efficacy of fingolimod.
Notes: Somers, V (reprint author),Hasselt Univ, Biomed Res Inst, Diepenbeek, Belgium veerle.somers@uhasselt.be
URI: http://hdl.handle.net/1942/17931
DOI: 10.1371/journal.pone.0111115
ISI #: 000343943700063
ISSN: 1932-6203
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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