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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/17857

Title: Differential response of Arabidopsis leaves and roots to cadmium: Glutathione-related chelating capacity vs antioxidant capacity
Authors: Jozefczak, Marijke
Keunen, Els
Schat, Henk
Bliek, Mattijs
Hernandez, Luis E.
Carleer, Robert
Remans, Tony
Bohler, Sacha
Vangronsveld, Jaco
Cuypers, Ann
Issue Date: 2014
Abstract: This study aims to uncover the spatiotemporal involvement of glutathione (GSH) in two major mechanisms of cadmium (Cd)-induced detoxification (i.e. chelation and antioxidative defence). A kinetic study was conducted on hydroponically grown Arabidopsis thaliana (L Heyhn) to gain insight into the early events after exposure to Cd. Cadmium detoxification was investigated at different levels, including gene transcripts, enzyme activities and metabolite content. Data indicate a time-dependent response both within roots and between plant organs. Early on in roots, GSH was preferentially allocated to phytochelatin (PC) synthesis destined for Cd chelation. This led to decreased GSH levels, without alternative pathways activated to complement GSH's antioxidative functions. After one day however, multiple antioxidative pathways increased including superoxide dismutase (SOD), ascorbate (AsA) and catalase (CAT) to ensure efficient neutralization of Cd-induced reactive oxygen species (ROS). As a consequence of Cd retention and detoxification in roots, a delayed response occurred in leaves. Together with high leaf thiol contents and possibly signalling responses from the roots, the leaves were protected, allowing them sufficient time to activate their defence mechanisms. (C) 2014 Elsevier Masson SAS. All rights reserved.
Notes: [Jozefczak, Marijke; Keunen, Els; Carleer, Robert; Remans, Tony; Bohler, Sacha; Vangronsveld, Jaco; Cuypers, Ann] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. [Schat, Henk; Bliek, Mattijs] Free Univ Amsterdam, Inst Mol & Cellular Biol, NL-1081 HV Amsterdam, Netherlands. [Hernandez, Luis E.] Univ Autonoma Madrid, Dept Biol, Lab Fisiol Vegetal, E-28049 Madrid, Spain.
URI: http://hdl.handle.net/1942/17857
DOI: 10.1016/j.plaphy.2014.07.001
ISI #: 000343349100001
ISSN: 0981-9428
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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