Document Server@UHasselt >
Research >
Research publications >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/17127

Title: Gene expression profiles reveal distinct immunological responses of cobalt and cerium dioxide nanoparticles in two in vitro lung epithelial cell models
Authors: Verstraelen, Sandra
Remy, Sylvie
Casals, Eudald
DE BOEVER, Patrick
Witters, Hilda
Gatti, Antonietta
Puntes, Victor
Nelissen, Inge
Issue Date: 2014
Citation: TOXICOLOGY LETTERS, 228 (3), p. 157-169
Abstract: Fragmentary knowledge exists on cellular signaling responses underlying possible adverse health effects of CoO- and CeO2-nanoparticles (NP)s after inhalation. We aimed to perform a time kinetic study of gene expression profiles induced by these NPs in alveolar A549 and bronchial BEAS-2B epithelial cells, and investigated possible immune system modulation. The kinetics of the cell responses induced by the NPs were different between the lung epithelial models. Both CoO- and CeO2-NP exposure induced mainly downregulation of gene transcription. BEAS-2B cells were found to be more sensitive, as they showed a higher number of differentially expressed transcripts (DET) at a 10-fold lower NP-concentration than A549 cells. Hierarchical clustering of all DET indicated that the transcriptional responses were heterogeneous among the two cell types and two NPs. Between 1% and 14% DET encoding markers involved in immune processes were observed. The transcriptional impact of the metal oxide NPs appeared to be cell-dependent, both at the general and immune response level, whereas each lung epithelial cell model responded differently to the two NP types. Thus, the study provides gene expression markers and immune processes involved in CoO- and CeO2-NP-induced toxicity, and demonstrates the usefulness of comprehensive-omics studies to differentiate between NP responses. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
Notes: [Verstraelen, Sandra; Remy, Sylvie; De Boever, Patrick; Witters, Hilda; Nelissen, Inge] Flemish Inst Technol Res VITO NV, Environm Risk & Hlth Unit, B-2400 Mol, Belgium. [Casals, Eudald; Puntes, Victor] ICN, Barcelona, Spain. [Gatti, Antonietta] Univ Modena & Reggio Emilia, Lab Biomat, Modena, Italy. [De Boever, Patrick] Hasselt Univ, Ctr Environm Sci, Diepenbeek, Belgium. sandra.verstraelen@vito.be; sylvie.remy@vito.be; eudald.casals@icn.cat; patrick.deboever@vito.be; hilda.witters@vito.be; gatti@nanodiagnostics.it; victor.puntes@icn.cat; inge.nelissen@vito.be
URI: http://hdl.handle.net/1942/17127
DOI: 10.1016/j.toxlet.2014.05.006
ISI #: 000339537400004
ISSN: 0378-4274
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

Files in This Item:

Description SizeFormat
published version2.05 MBAdobe PDF

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.