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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/17111

Title: Construction of helper plasmid-mediated dual-display phage for autoantibody screening in serum
Authors: RAJARAM, Kaushik
VERMEEREN, Veronique
SOMERS, Klaartje
SOMERS, Veerle
Issue Date: 2014
Publisher: SPRINGER
Abstract: M13 filamentous bacteriophage has been used in displaying disease-specific antibodies, biomarkers, and peptides. One of the major drawbacks of using phage in diagnostic assays is the aspecific adsorption of proteins leading to a high background signal and decreasing sensitivity. To deal with this, we developed a genetically pure, exchangeable dual-display phage system in which biomarkers and streptavidin-binding protein (SBP) are displayed at opposite ends of the phage. This approach allows for sample purification, using streptavidin-coated magnetic beads resulting in a higher sensitivity of signal detection assays. Our dual-display cassette system approach also allows for easy exchange of both the anchor protein (SBP) and the displayed biomarker. The presented principle is applied for the detection of antibody reactivity against UH-RA. 21 which is a good candidate biomarker for rheumatoid arthritis (RA). The applicability of dual-display phage preparation using a helper plasmid system is demonstrated, and its increased sensitivity in phage ELISA assays using patient serum samples is shown.
Notes: [Rajaram, Kaushik; Vermeeren, Veronique; Somers, Veerle; Michiels, Luc] Hasselt Univ, Biomed Res Inst, B-3500 Hasselt, Belgium. [Somers, Klaartje] Complix NV, BioVille, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/17111
DOI: 10.1007/s00253-014-5713-8
ISI #: 000338237400017
ISSN: 0175-7598
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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