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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/17110

Title: Metabolite profiling and peptidoglycan analysis of transient cell wall-deficient bacteria in a new Escherichia coli model system.
Authors: Cambré, A.
Zimmerman, M.
Sauer, U.
Vivijs, B.
Cenens, W.
Michiels, C.W.
Aertsen, A.
Loessner, M.J.
NOBEN, Jean-Paul
Ayala, J.A.
Lavigne, R.
Briers, Y.
Issue Date: 2015
Citation: ENVIRONMENTAL MICROBIOLOGY, 17 (5), p. 1586-1599
Abstract: Many bacteria are able to assume a transient cell wall-deficient (or L-form) state under favorable osmotic conditions. Cell wall stress such as exposure to β-lactam antibiotics can enforce the transition to and maintenance of this state. L-forms actively proliferate and can return to the walled state upon removal of the inducing agent. We have adopted Escherichia coli as a model system for the controlled transition to and reversion from the L-form state, and have studied these dynamics with genetics, cell biology and ‘omics’ technologies. As such, a transposon mutagenesis screen underscored the requirement for the Rcs phosphorelay and colanic acid synthesis, while proteomics show only little differences between rods and L-forms. In contrast, metabolome comparison reveals the high abundance of lysophospholipids and phospholipids with unsaturated or cyclopropanized fatty acids in E. coli L-forms. This increase of membrane lipids associated with increased membrane fluidity may facilitate proliferation through bud formation. Visualization of the residual peptidoglycan with a fluorescently labeled peptidoglycan binding protein indicates de novo cell wall synthesis and a role for septal peptidoglycan synthesis during bud constriction. The DD-carboxypeptidases PBP5 and PBP6 are three- and four-fold upregulated in L-forms, indicating a specific role for regulation of crosslinking during L-form proliferation.
Notes: Briers, Y (reprint author), Katholieke Univ Leuven, Lab Gene Technol, Dept Biosyst, B-3001 Heverlee, Belgium. yves.briers@kuleuven.be
URI: http://hdl.handle.net/1942/17110
DOI: 10.1111/1462-2920.12594
ISI #: 000353507100010
ISSN: 1462-2912
Category: A1
Type: Journal Contribution
Validation: ecoom, 2016
Appears in Collections: Research publications

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