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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/17084

Title: Calorie restriction-induced changes in the secretome of human adipocytes, comparison with resveratrol-induced secretome effects.
Authors: RENES, J.
Rosenow, A.
Roumans, N.
NOBEN, Jean-Paul
Mariman, E.C.
Issue Date: 2014
Citation: BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1844 (9), p. 1511-1522
Abstract: Obesity is characterized by dysfunctional white adipose tissue (WAT) that ultimately may lead to metabolic diseases. Calorie restriction (CR) reduces the risk for age and obesity-associated complications. The impact of CR on obesity has been examined with human intervention studies, which showed alterations in circulating adipokines. However, a direct effect of CR on the human adipocyte secretome remains elusive. Therefore, the effect of a 96 h low glucose CR on the secretion profile of in vitro cultured mature human SGBS adipocytes was investigated by using proteomics technology. Low-glucose CR decreased the adipocyte triglyceride contents and resulted in an altered secretion profile. Changes in the secretome indicated an improved inflammatory phenotype. In addition, several adipocyte-secreted proteins related to insulin resistance showed a reversed expression after low-glucose CR. Furthermore, 6 novel CR-regulated adipocyte-secreted proteins were identified. Since resveratrol (RSV) mimics CR we compared results from this study with data from our previous RSV study on the SGBS adipocyte secretome. The CR and RSV adipocyte secretomes partly differed from each other, although both treatment strategies lead to secretome changes indicating a less inflammatory phenotype. Furthermore, both treatments induced SIRT1 expression and resulted in a reversed expression of detrimental adipokines associated with metabolic complications.
Notes: Renes, J (reprint author),Maastricht Univ, Dept Human Biol, POB 616, NL-6200 MD Maastricht, Netherlands. j.renes@maastrichtuniversity.nl
URI: http://hdl.handle.net/1942/17084
DOI: 10.1016/j.bbapap.2014.04.023
ISI #: 000340339500010
ISSN: 1570-9639
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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