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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/17081

Title: Molecular characterization of three Lactobacillus delbrueckii subsp. bulgaricus phages.
Authors: Casey, E.
Mahony, J.
O'Connell-Motherway, M.
Bottacini, F.
Cornelissen, A.
Neve, H.
Heller, K.J.
NOBEN, Jean-Paul
Dal Bello, F.
van Sinderen, D.
Issue Date: 2014
Abstract: In this study, three phages infecting Lactobacillus delbrueckii subsp. bulgaricus, named Ld3, Ld17, and Ld25A, were isolated from whey samples obtained from various industrial fermentations. These phages were further characterized in a multifaceted approach: (i) biological and physical characterization through host range analysis and electron microscopy; (ii) genetic assessment through genome analysis; (iii) mass spectrometry analysis of the structural components of the phages; and (iv), for one phage, transcriptional analysis by Northern hybridization, reverse transcription-PCR, and primer extension. The three obtained phage genomes display high levels of sequence identity to each other and to genomes of the so-called group b L. delbrueckii phages c5, LL-Ku, and phiLdb, where some of the observed differences are believed to be responsible for host range variations.
Notes: van Sinderen, D (reprint author), Natl Univ Ireland Univ Coll Cork, Sch Microbiol, Cork, Ireland. d.vansinderen@ucc.ie
URI: http://hdl.handle.net/1942/17081
Link to publication: PubMed PMID: 25002431
DOI: 10.1128/AEM.01268-14
ISI #: 000341488200008
ISSN: 0099-2240
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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