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|Title: ||Molecular characterization of three Lactobacillus delbrueckii subsp. bulgaricus phages.|
|Authors: ||Casey, E.|
Dal Bello, F.
van Sinderen, D.
|Issue Date: ||2014|
|Citation: ||APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80 (18), p. 5623-5635|
|Abstract: ||In this study, three phages infecting Lactobacillus delbrueckii subsp. bulgaricus, named Ld3, Ld17, and Ld25A, were isolated from whey samples obtained from various industrial fermentations. These phages were further characterized in a multifaceted approach: (i) biological and physical characterization through host range analysis and electron microscopy; (ii) genetic assessment through genome analysis; (iii) mass spectrometry analysis of the structural components of the phages; and (iv), for one phage, transcriptional analysis by Northern hybridization, reverse transcription-PCR, and primer extension. The three obtained phage genomes display high levels of sequence identity to each other and to genomes of the so-called group b L. delbrueckii phages c5, LL-Ku, and phiLdb, where some of the observed differences are believed to be responsible for host range variations.|
|Notes: ||van Sinderen, D (reprint author), Natl Univ Ireland Univ Coll Cork, Sch Microbiol, Cork, Ireland.
|Link to publication: ||PubMed PMID: 25002431|
|ISI #: ||000341488200008|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2015|
|Appears in Collections: ||Research publications|
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