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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/17034

Title: CXCL5 as a potential novel prognostic factor in early stage non-small cell lung cancer: results of a study of expression levels of 23 genes
Authors: Kowalczuk, Oksana
BURZYKOWSKI, Tomasz
Niklinska, Wieslawa Ewa
Kozlowski, Miroslaw
Chyczewski, Lech
Niklinski, Jacek
Issue Date: 2014
Citation: TUMOR BIOLOGY, 35 (5), p. 4619-4628
Abstract: As the current staging system is imprecise for estimating prognosis of early stage non-small cell lung cancer (NSCLC), it is important to identify other methods for selecting high-risk patients after failed surgical treatment. The aim of the study was to evaluate the expression of 23 genes as putative prognostic markers in early stage NSCLC. The study was performed on 109 pairs of tumor and matched unaffected lung tissue surgical specimens taken from stage I and II NSCLC patients. We evaluated the mRNA level of 23 genes using the real-time PCR method. The difference in the expression between the tumor and normal tissue for each gene was analyzed using a general linear model. The influence of gene expression on survival was analyzed by using the proportional hazards model. Eighteen out of the 23 genes showed statistically significant differences in expression between the tumor and non-tumor tissue. For 12 genes (ITGB1, ITGB3, CXCL1, CXCL8, CXCL9, CXCL10, CXCL11, CXCR3, CXCR4, TNF, CHKA, AGFG1, and CTC1), the expression was lower, and for six genes (ITGA5, IL8, IL6, CXCL2, CXCL3, and CXCL12), it was higher in the tumor tissue as compared to the matched normal tissue. Expression changes were more pronounced in squamous cell carcinomas than in adenocarcinomas or large cell carcinomas. Of all the analyzed genes, only CXCL5 was found to statistically significantly (p = 0.04) influence both overall and disease-free survival. Among the 23 genes previously suggested to be relevant for early staged NSCLC patients’ postoperative outcome, only CXCL5 showed a statistically significant prognostic effect.
Notes: Niklinski, J (reprint author), Med Univ Bialystok, Dept Clin Mol Biol, 13 Waszyngtona St, PL-15267 Bialystok, Poland. niklinsj@umb.edu.pl
URI: http://hdl.handle.net/1942/17034
DOI: 10.1007/s13277-014-1605-x
ISI #: 000335759800077
ISSN: 1010-4283
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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