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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16987

Title: Selective Identification of Macrophages and Cancer Cells Based on Thermal Transport through Surface-Imprinted Polymer Layers
Authors: Eersels, Kasper
van Grinsven, Bart
Ethirajan, Anitha
Timmermans, Silke
Monroy, Kathia L. Jimenez
Bogie, Jeroen F. J.
Punniyakoti, Sathya
Vandenryt, Thijs
Hendriks, Jerome J. A.
Cleij, Thomas J.
Daemen, Mat J. A. P.
Somers, Veerle
De Ceuninck, Ward
Wagner, Patrick
Issue Date: 2013
Citation: ACS APPLIED MATERIALS & INTERFACES, 5 (15), p. 7258-7267
Abstract: In this article, we describe a novel straightforward method for the specific identification of viable cells (macrophages and cancer cell lines MCF-7 and Jurkat) in a buffer solution. The detection of the various cell types is based on changes of the heat transfer resistance at the solid-liquid interface of a thermal sensor device induced by binding of the cells to a surface-imprinted polymer layer covering an aluminum chip. We observed that the binding of cells to the polymer layer results in a measurable increase of heat transfer resistance, meaning that the cells act as a thermally insulating layer. The detection limit was found to be on the order of 10(4) cells/mL, and mutual cross-selectivity effects between the cells and different types of imprints were carefully characterized. Finally, a rinsing method was applied, allowing for the specific detection of cancer cells with their respective imprints while the cross-selectivity toward peripheral blood mononuclear cells was negligible. The concept of the sensor platform is fast and low-cost while allowing also for repetitive measurements.
Notes: [Eersels, Kasper; van Grinsven, Bart; Ethirajan, Anitha; Monroy, Kathia L. Jimenez; Punniyakoti, Sathya; Cleij, Thomas J.; De Ceuninck, Ward; Wagner, Patrick] Hasselt Univ, Inst Mat Res IMO, B-3590 Diepenbeek, Belgium. [Timmermans, Silke; Bogie, Jeroen F. J.; Hendriks, Jerome J. A.; Somers, Veerle] Hasselt Univ, Inst Biomed Res BIOMED, B-3590 Diepenbeek, Belgium. [Vandenryt, Thijs] XIOS Univ Coll, Dept Appl Engn, B-3590 Diepenbeek, Belgium. [Cleij, Thomas J.] Maastricht Univ, Maastricht Sci Programme, NL-6200 MD Maastricht, Netherlands. [Daemen, Mat J. A. P.] Univ Amsterdam, Acad Med Ctr, NL-1105 AZ Amsterdam, Netherlands. [De Ceuninck, Ward; Wagner, Patrick] IMEC Vzw, IMOMEC Div, B-3590 Diepenbeek, Belgium.
URI: http://hdl.handle.net/1942/16987
DOI: 10.1021/am401605d
ISI #: 000323241100068
ISSN: 1944-8244
Category: A1
Type: Journal Contribution
Validation: ecoom, 2014
Appears in Collections: Research publications

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