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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16914

Title: Zymography methods for visualizing hydrolytic enzymes
Authors: Vandooren, Jennifer
Geurts, Nathalie
Martens, Erik
Van den Steen, Philippe E.
OPDENAKKER, Ghislain
Issue Date: 2013
Publisher: NATURE PUBLISHING GROUP
Citation: NATURE METHODS, 10 (3), p. 211-220
Abstract: Zymography is a technique for studying hydrolytic enzymes on the basis of substrate degradation. It is a powerful., but often misinterpreted, tool. yielding information on potential. hydrolytic activities, enzyme forms and the locations of active enzymes. In this Review, zymography techniques are compared in terms of advantages, limitations and interpretations. With in gel zymography, enzyme forms are visualized according to their molecular weights. Proteolytic activities are localized in tissue sections with in situ zymography. In vivo zymography can pinpoint proteolytic activity to sites in an intact organism. Future development of novel. substrate probes and improvement in detection and imaging methods will increase the applicability of zymography for (reverse) degradomics studies.
Notes: [Vandooren, Jennifer; Geurts, Nathalie; Martens, Erik; Van den Steen, Philippe E.; Opdenakker, Ghislain] Univ Louvain, KU Leuven, Rega Inst Med Res, Immunobiol Lab, Louvain, Belgium.
URI: http://hdl.handle.net/1942/16914
DOI: 10.1038/NMETH.2371
ISI #: 000316650800016
ISSN: 1548-7091
Category: A1
Type: Journal Contribution
Appears in Collections: Research publications

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