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|Title: ||HLA-E restricted CD8+ T cell subsets are phenotypically altered in multiple sclerosis patients.|
|Authors: ||Pannemans, Kim|
Van Wijmeersch, Bart
|Issue Date: ||2014|
|Citation: ||MULTIPLE SCLEROSIS JOURNAL, 20 (7), p. 790-801|
|Abstract: ||Background: The importance of Qa-1 restricted CD8+ T cells in regulating autoreactive T cell responses has been demonstrated in animal models for autoimmune disorders, including multiple sclerosis (MS).
Objective: We hypothesize that their human variant, HLA-E restricted CD8+ T cells, fulfills a similar regulatory role in man and that these cells are of importance in MS.
Methods: A large cohort of MS patients and healthy controls was genotyped for the two known HLA-E polymorphisms. Flow cytometry was used to determine HLA-E expression kinetics and to phenotype HLA-E restricted CD8+ T cells. Immunohistochemistry was performed to investigate HLA-E expression in the central nervous system (CNS) of MS patients.
Results: HLA-E is upregulated on immune cells upon in vitro activation and this upregulation is polymorphism-dependent for T and B cells. T and B cells in lesions of MS patients show enhanced HLA-E expression. Furthermore, NKG2C+CD8+ T cells of MS patients have a significantly lower Foxp3 expression, while NKG2A+CD8+ T cells of MS patients produce higher levels of pro-inflammatory cytokines compared to those of healthy individuals.
Conclusion: Our study indicates that the HLA-E system is altered in MS and could play a regulatory role in disease.|
|Notes: ||[Pannemans, Kim; Broux, Bieke; Broekmans, Tom; Van Wijmeersch, Bart; Stinissen, Piet; Hellings, Niels] Hasselt Univ, Biomed Onderzoeksinst, B-3590 Diepenbeek, Belgium. [Goris, An; Dubois, Benedicte] KULeuven, Lab Neuroimmunol, Leuven, Belgium. [Broekmans, Tom] PHL Univ Coll, Dept Hlth Care, Hasselt, Belgium. [Geraghty, Daniel] Fred Hutchinson Canc Res Ctr, Clin Res Div, Seattle, WA USA.|
|ISI #: ||000337854400006|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2015|
|Appears in Collections: ||Research publications|
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