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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16878

Title: HLA-E restricted CD8+ T cell subsets are phenotypically altered in multiple sclerosis patients.
Authors: Pannemans, Kim
Broux, Bieke
Goris, An
Dubois, Bénédicte
Broekmans, Tom
Van Wijmeersch, Bart
Geraghty, Daniel
Stinissen, Piet
Hellings, Niels
Issue Date: 2014
Citation: MULTIPLE SCLEROSIS JOURNAL, 20 (7), p. 790-801
Abstract: Background: The importance of Qa-1 restricted CD8+ T cells in regulating autoreactive T cell responses has been demonstrated in animal models for autoimmune disorders, including multiple sclerosis (MS). Objective: We hypothesize that their human variant, HLA-E restricted CD8+ T cells, fulfills a similar regulatory role in man and that these cells are of importance in MS. Methods: A large cohort of MS patients and healthy controls was genotyped for the two known HLA-E polymorphisms. Flow cytometry was used to determine HLA-E expression kinetics and to phenotype HLA-E restricted CD8+ T cells. Immunohistochemistry was performed to investigate HLA-E expression in the central nervous system (CNS) of MS patients. Results: HLA-E is upregulated on immune cells upon in vitro activation and this upregulation is polymorphism-dependent for T and B cells. T and B cells in lesions of MS patients show enhanced HLA-E expression. Furthermore, NKG2C+CD8+ T cells of MS patients have a significantly lower Foxp3 expression, while NKG2A+CD8+ T cells of MS patients produce higher levels of pro-inflammatory cytokines compared to those of healthy individuals. Conclusion: Our study indicates that the HLA-E system is altered in MS and could play a regulatory role in disease.
Notes: [Pannemans, Kim; Broux, Bieke; Broekmans, Tom; Van Wijmeersch, Bart; Stinissen, Piet; Hellings, Niels] Hasselt Univ, Biomed Onderzoeksinst, B-3590 Diepenbeek, Belgium. [Goris, An; Dubois, Benedicte] KULeuven, Lab Neuroimmunol, Leuven, Belgium. [Broekmans, Tom] PHL Univ Coll, Dept Hlth Care, Hasselt, Belgium. [Geraghty, Daniel] Fred Hutchinson Canc Res Ctr, Clin Res Div, Seattle, WA USA.
URI: http://hdl.handle.net/1942/16878
DOI: 10.1177/1352458513509703
ISI #: 000337854400006
ISSN: 1352-4585
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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