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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16825

Title: Heat-Transfer Resistance Measurement Method (HTM)-Based Cell Detection at Trace Levels Using a Progressive Enrichment Approach with Highly Selective Cell-Binding Surface Imprints
Authors: Bers, Karolien
EERSELS, Kasper
VAN GRINSVEN, Bart
Daemen, Mat
BOGIE, Jeroen
Hendriks, Jerome
Bouwmans, Evelien E.
Püttmann, Christiane
Stein, Christoph
Barth, Stefan
Bos, Gerard M. J.
GERMERAAD, Wilfred
DE CEUNINCK, Ward
WAGNER, Patrick
Issue Date: 2014
Citation: LANGMUIR, 30 (12), p. 3631-3639
Abstract: Surface-imprinted polymers allow for specific cell detection based on simultaneous recognition of the cell shape, cell size, and cell membrane functionalities by macromolecular cell imprints. In this study, the specificity of detection and the detection sensitivity for target cells within a pool of non-target cells were analyzed for a cell-specific surface-imprinted polymer combined with a heat-transfer-based read-out technique (HTM). A modified Chinese hamster ovarian cell line (CHO-ldlD) was used as a model system on which the transmembrane protein mucin-1 (MUC1) could be excessively expressed and for which the occurrence of MUC1 glycosylation could be controlled. In specific cancer cells, the overexpressed MUC1 protein typically shows an aberrant apical distribution and glycosylation. We show that surface-imprinted polymers discriminate between cell types that (1) only differ in the expression of a specific membrane protein (MUC1) or (2) only differ in the membrane protein being glycosylated or not. Moreover, surface-imprinted polymers of cells carrying different glycoforms of the same membrane protein do target both types of cells. These findings illustrate the high specificity of cell detection that can be reached by the structural imprinting of cells in polymer layers. Competitiveness between target and non-target cells was proven to negatively affect the detection sensitivity of target cells. Furthermore, we show that the detection sensitivity can be increased significantly by repetitively exposing the surface to the sample and eliminating non-specifically bound cells by flushing between consecutive cell exposures.
URI: http://hdl.handle.net/1942/16825
DOI: 10.1021/la5001232
ISI #: 000333776400043
ISSN: 0743-7463
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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