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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16795

Title: Mast cells protect from post-traumatic spinal cord damage in mice by degrading inflammation-associated cytokines via mouse mast cell protease 4
Authors: NELISSEN, Sofie
VANGANSEWINKEL, Tim
GEURTS, Nathalie
GEBOES, Lies
LEMMENS, Evi
VIDAL VERA, Pia
LEMMENS, Stefanie
WILLEMS, Leen
Boato, Francesco
DOOLEY, Dearbhaile
Pehl, Debora
Pejler, Gunnar
Maurer, Marcus
Metz, Martin
HENDRIX, Sven
Issue Date: 2014
Citation: NEUROBIOLOGY OF DISEASE, 62, p. 260-272
Abstract: Mast cells (MCs) are found abundantly in the central nervous system and play a complex role in neuroinflammatory diseases such as multiple sclerosis and stroke. In the present study,we show thatMC-deficient KitW-sh/W-sh mice display significantly increased astrogliosis and T cell infiltration as well as significantly reduced functional recovery after spinal cord injury compared to wildtype mice. In addition, MC-deficient mice show significantly increased levels of MCP-1, TNF-α, IL-10 and IL-13 protein levels in the spinal cord.Mice deficient inmouse mast cell protease 4 (mMCP4), an MC-specific chymase, also showed increased MCP-1, IL-6 and IL-13 protein levels in spinal cord samples and a decreased functional outcome after spinal cord injury. A degradation assay using supernatant from MCs derived from either mMCP4−/− mice or controls revealed that mMCP4 cleaves MCP-1, IL-6, and IL-13 suggesting a protective role for MC proteases in neuroinflammation. These data show for the first time that MCs may be protective after spinal cord injury and that they may reduce CNS damage by degrading inflammation-associated cytokines via the MC-specific chymasemMCP4.
URI: http://hdl.handle.net/1942/16795
DOI: 10.1016/j.nbd.2013.09.012
ISI #: 000330553600024
ISSN: 0969-9961
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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