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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/1679

Title: Compartmental Modeling Of The Fluorescence Anisotropy Decay Of A Cylindrically Symmetric Brownian Rotor: Identifiability Analysis.
Authors: Boens, N.
Novikov, E.
Issue Date: 2006
Publisher: Wiley
Citation: CHEMPHYSCHEM, 7. p. 2559-2566
Abstract: We present the results of the deterministic identifiability analysis based on similarity transformation for models of one-state excited-state events of cylindrically symmetric rotors in isotropic environments undergoing rotational diffusion described by Brownian reorientation. Such an analysis on error-free time-resolved fluorescence (anisotropy) data can reveal whether the parameters of the considered model can be determined. The fluorescence -response functions I||(t) and I(t), for fluorescence polarized respectively parallel and perpendicular to the electric vector of linearly polarized excitation, are used to construct, in convenient matrix form, expressions of the sum S(t)=I||(t)+2 I(t), the difference D(t)=I||(t)-I(t), and the time-resolved fluorescence anisotropy r(t)=D(t)/S(t). The identifiability analysis of r(t) demonstrates that the rotational diffusion coefficients D|| and D for rotation respectively about and perpendicular to the symmetry axis can be uniquely resolved. However, the polar and azimuthal angles defining the absorption and emission transition moments in the molecular reference frame are not individually identifiable. Nevertheless, the difference between the polar angles of these transition moments is uniquely determined.
URI: http://hdl.handle.net/1942/1679
DOI: 10.1002/cphc.200600309
ISI #: 000244334000021
ISSN: 1439-4235
Category: A1
Type: Journal Contribution
Validation: ecoom, 2008
Appears in Collections: Research publications

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