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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16760

Title: Characterization of the peripheral blood transcriptome in a repeated measures design using a panel of healthy individuals
Authors: DE BOEVER, Patrick
Wens, Britt
Forcheh, Anyiawung Chiara
Reynders, Hans
Nelen, Vera
Kleinjans, Jos
Van Larebeke, Nicolas
VERBEKE, Geert
VALKENBORG, Dirk
Schoeters, Greet
Issue Date: 2014
Citation: GENOMICS, 103 (1), p. 31-39
Abstract: A repeated measures microarray design with 22 healthy, non-smoking volunteers (aging 32 ± 5 years) was set up to study transcriptome profiles in whole blood samples. The results indicate that repeatable data can be obtained with highwithin-subject correlation. Probes that could discriminate between individuals are associated with immune and inflammatory functions. When investigating possible time trends in the microarray data, we have found no differential expression within a sampling period (within-season effect). Differential expression was observed between sampling seasons and the data suggest a weak response of genes related to immune system functioning. Finally, a high number of probes showed significant season-specific expression variability within subjects. Expression variability increased in springtime and there was an association of the probe list with immune system functioning. Our study suggests that the blood transcriptome of healthy individuals is reproducible over a time period of several months.
Notes: De Boever, P (reprint author), Flemish Inst Technol Res VITO, Unit Environm Risk & Hlth, Ind Vlasmeer 7, B-2400 Mol, Belgium patrick.deboever@vito.be
URI: http://hdl.handle.net/1942/16760
DOI: 10.1016/j.ygeno.2013.11.006
ISI #: 000333512800004
ISSN: 0888-7543
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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