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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16692

Title: Gene expressions changes in bronchial epithelial cells: Markers for respiratory sensitizers and exploration of the NRF2 pathway
Authors: Remy, Sylvie
Verstraelen, Sandra
Van Den Heuvel, Rosette
Nelissen, Inge
Lambrechts, Nathalie
Schoeters, Greet
Issue Date: 2014
Citation: TOXICOLOGY IN VITRO, 28 (2), p. 209-217
Abstract: For the classification of respiratory sensitizing chemicals, no validated in vivo nor in vitro tests are currently available. In this study, we evaluated whether respiratory sensitizers trigger specific signals in human bronchial epithelial (BEAS-2B) cells at the level of the transcriptome. The cells were exposed during 6, 10, and 24 h to 4 respiratory sensitizers and 6 non-respiratory sensitizers (3 skin sensitizers and 3 respiratory irritants) at a concentration inducing 20% cell viability loss after 24 h. Changes in gene expression were evaluated using Agilent Whole Human Genome 4 44 K oligonucleotide arrays. A limited number of 11 transcripts could be identified as potential biomarkers to identify respiratory sensitizers. Three of these transcripts are associated to immune system processes (HSPA5, UPP1, and SEPRINE1). In addition, the transcriptome was screened for transcripts that are differentially expressed compared to vehicle control for each chemical. The results show that the NRF2-mediated oxidative stress response is activated in the cell line after stimulation with all of the chemicals that were selected in our study, and that – at the level of gene expression – this pathway shows no potential to discriminate between any of the three compound groups: respiratory sensitizers, skin sensitizers, or electrophilic respiratory irritants.
Notes: Remy, S (reprint author), Flemish Inst Technol Res VITO NV, Unit Environm Risk & Hlth, Boeretang 200, B-2400 Mol, Belgium. sylvie.remy@vito.be; sandra.verstraelen@vito.be; rosette.vandenheuvel@vito.be; inge.nelissen@vito.be; nathalie.lambrechts@vito.be; jef.hooyberghs@vito.be; greet.schoeters@vito.be
URI: http://hdl.handle.net/1942/16692
DOI: 10.1016/j.tiv.2013.10.017
ISI #: 000331029200009
ISSN: 0887-2333
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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