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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16684

Title: An impedimetric immunosensor based on diamond nanowires decorated with nickel nanoparticles
Authors: Subramanian, Palaniappan
Motorina, Anastasiia
YEAP, Weng Siang
Coffinier, Yannick
Zaitsev, Vladimir
Niedziolka-Jonsson, Joanna
Boukherroub, Rabah
Szunerits, Sabine
Issue Date: 2014
Citation: ANALYST, 139 (7), p. 1726-1731
Abstract: Nanostructured boron-doped diamond has been investigated as a sensitive impedimetric electrode for the detection of immunoglobulin G (IgG). The immunosensor was constructed in a three-step process: (i) reactive ion etching of flat boron-doped diamond (BDD) interfaces to synthesize BDD nanowires (BDD NWs), (ii) electrochemical deposition of nickel nanoparticles (Ni NPs) on the BDD NWs, and (iii) immobilization of biotin-tagged anti-IgG onto the Ni NPs. Electrochemical impedance spectroscopy (EIS) was used to follow the binding of IgG at different concentrations without the use of any additional label. A detection limit of 0.3 ng mL−1 (2 nM) with a dynamic range up to 300 ng mL−1 (2 μM) was obtained with the interface. Moreover, the study demonstrated that this immunosensor exhibits good stability over time and allows regeneration by incubation in ethylenediaminetetraacetic acid (EDTA) aqueous solution.
Notes: Szunerits, S (reprint author), Univ Lille 1, CNRS USR 3078, IRI, Parc Haute Borne,50 Ave Halley,BP 70478, F-59658 Villeneuve Dascq, France.
URI: http://hdl.handle.net/1942/16684
DOI: 10.1039/c3an02045b
ISI #: 000332422400021
ISSN: 0003-2654
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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