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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16666

Title: Combining Electrochemical Impedance Spectroscopy and Surface Plasmon Resonance into one Simultaneous Read-Out System for the Detection of Surface Interactions
Authors: VANDENRYT, Thijs
Pohl, Andrea
VAN GRINSVEN, Bart
THOELEN, Ronald
DE CEUNINCK, Ward
WAGNER, Patrick
Opitz, Jörg
Issue Date: 2013
Citation: SENSORS, 13 (11), p. 14650-14661
Abstract: In this article we describe the integration of impedance spectroscopy (EIS) and surface plasmon resonance (SPR) into one surface analytic device. A polydimethylsiloxane (PDMS) flow cell is created, matching the dimensions of a commercially available sensor chip used for SPR measurements. This flow cell allowed simultaneous measurements between an EIS and a SPR setup. After a successful integration, a proof of principle study was conducted to investigate any signs of interference between the two systems during a measurement. The flow cell was rinsed with 10 mM Tris-HCl and 1× PBS buffer in an alternating manner, while impedance and shifts of the resonance angle were monitored. After achieving a successful proof of principle, a usability test was conducted. It was assessed whether simultaneous detection occurred when: (i) Protein A is adsorbed to the gold surface of the chip; (ii) The non-occupied zone is blocked with BSA molecules and (iii) IgG1 is bound to the Protein A. The results indicate a successful merge between SPR and EIS.
URI: http://hdl.handle.net/1942/16666
DOI: 10.3390/s131114650
ISI #: 000330321100023
ISSN: 1424-8220
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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