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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16580

Title: Placental DNA hypomethylation in assiociation with particulate air pollution in early life
Authors: Janssen, Bram G.
Godderis, Lode
Pieters, Nicky
Poels, Katrien
Kicinski, Michal
Cuypers, Ann
Fierens, Frans
Penders, Joris
Plusquin, Michelle
Gyselaers, Wilfried
Nawrot, Tim S.
Issue Date: 2013
Citation: Particle and Fibre Toxicology, 10 (22), p. 1-11
Abstract: Background There is evidence that altered DNA methylation is an important epigenetic mechanism in prenatal programming and that developmental periods are sensitive to environmental stressors. We hypothesized that exposure to fine particles (PM2.5) during pregnancy could influence DNA methylation patterns of the placenta. Methods In the ENVIRONAGE birth cohort, levels of 5’-methyl-deoxycytidine (5-mdC) and deoxycytidine (dC) were quantified in placental DNA from 240 newborns. Multiple regression models were used to study placental global DNA methylation and in utero exposure to PM2.5 over various time windows during pregnancy. Results PM2.5 exposure during pregnancy averaged (25th-75th percentile) 17.4 (15.4-19.3) μg/m3. Placental global DNA methylation was inversely associated with PM2.5 exposures during whole pregnancy and relatively decreased by 2.19% (95% confidence interval [CI]: -3.65, -0.73%, p = 0.004) for each 5 μg/m3 increase in exposure to PM2.5. In a multi-lag model in which all three trimester exposures were fitted as independent variables in the same regression model, only exposure to PM2.5 during trimester 1 was significantly associated with lower global DNA methylation (-2.13% per 5 μg/m3 increase, 95% CI: -3.71, -0.54%, p = 0.009). When we analyzed shorter time windows of exposure within trimester 1, we observed a lower placental DNA methylation at birth during all implantation stages but exposure during the implantation range (6-21d) was strongest associated (-1.08% per 5 μg/m3 increase, 95% CI: -1.80, -0.36%, p = 0.004). Conclusions We observed a lower degree of placental global DNA methylation in association with exposure to particulate air pollution in early pregnancy, including the critical stages of implantation. Future studies should elucidate genome-wide and gene-specific methylation patterns in placental tissue that could link particulate exposure during in utero life and early epigenetic modulations.
Notes: [Janssen, Bram G.; Pieters, Nicky; Kicinski, Michal; Cuypers, Ann; Plusquin, Michelle; Nawrot, Tim S.] Hasselt Univ, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. [Godderis, Lode; Poels, Katrien; Nawrot, Tim S.] Leuven Univ KU Leuven, Dept Publ Hlth Occupat Environm & Insurance Med, Louvain, Belgium. [Godderis, Lode] Idewe, External Serv Prevent & Protect Work, Heverlee, Belgium. [Fierens, Frans] Belgian Interreg Environm Agcy, Brussels, Belgium. [Penders, Joris] East Limburg Hosp, Lab Clin Biol, Genk, Belgium. [Penders, Joris; Gyselaers, Wilfried] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. [Gyselaers, Wilfried] East Limburg Hosp, Dept Obstet, Genk, Belgium.
URI: http://hdl.handle.net/1942/16580
DOI: 10.1186/1743-8977-10-22
ISI #: 000323545300001
ISSN: 1743-8977
Category: A1
Type: Journal Contribution
Validation: ecoom, 2014
Appears in Collections: Research publications

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