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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16431

Title: Mesenchymal stem cell secreted platelet derived growth factor exerts a pro-migratory effect on resident Cardiac Atrial appendage Stem Cells
Authors: WINDMOLDERS, Severina
De Boeck, Astrid
KONINCKX, Remco
Daniëls, Annick
De Weyer, Olivier
Bracke, Marc
HENDRIKX, Marc
HENSEN, Karen
RUMMENS, Jean-Luc
Issue Date: 2014
Citation: JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 66, p. 177-188
Abstract: Mesenchymal stem cells (MSCs) modulate cardiac healing after myocardial injury through the release of paracrine factors, but the exact mechanisms are still unknown. One possible mechanism is through mobilization of endogenous cardiac stemcells (CSCs). This study aimed to test the pro-migratory effect ofMSC conditionedmedium (MSC-CM) on endogenous CSCs from human cardiac tissue. By using a three-dimensional collagen assay, we found that MSC-CM improved migration of cells from human cardiac tissue. Cell counts, perimeter and area measurements were utilized to quantify migration effects. To examine whether resident stem cells were among the migrating cells, specific stem cell properties were investigated. The migrating cells displayed strong similarities with resident Cardiac Atrial appendage Stem Cells (CASCs), including a clonogenic potential of~21.5% and expression of pluripotency associated genes like Oct-4, Nanog, c-Myc and Klf-4. Similar to CASCs,migrating cells demonstrated high aldehyde dehydrogenase activity and were able to differentiate towards cardiomyocytes. Receptor tyrosine kinase analysis and collagen assays performed with recombinant platelet derived growth factor (PDGF)-AA and ImatinibMesylate, a PDGF receptor inhibitor, suggested a role for the PDGFAA/PDGF receptor α axis in enhancing the migration process of CASCs. In conclusion, our findings demonstrate that factors present in MSC-CM improve migration of resident stem cells from human cardiac tissue. These data open doors towards future therapies inwhichMSC secreted factors, like PDGF-AA, can be utilized to enhance the recruitment of CASCs towards the site of myocardial injury.
Notes: Rummens, JL (reprint author),Jessa Hosp, Lab Expt Hematol, Campus Virga Jesse,Stadsomvaart 11, B-3500 Hasselt, Belgium severina.windmolders@uhasselt.be; adeboeck@ucalgary.ca; remco.koninckx@jessazh.be; annick.daniels@jessazh.be; olivier.dewever@ugent.be; marcl.bracke@ugent.be; marc.hendrilor@jessazh.be; karen.hensen@jessazh.be; jean-luc.rummens@jessazh.be
URI: http://hdl.handle.net/1942/16431
DOI: 10.1016/j.yjmcc.2013.11.016
ISI #: 000330080700019
ISSN: 0022-2828
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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