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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16429

Title: Sensitive in vivo cell detection using size-optimized superparamagnetic nanoparticles
Authors: Trekker, Jesse
Leten, Cindy
STRUYS, Tom
Lazenka, Vera V.
Argibay, Barbara
Micholt, Liesbeth
LAMBRICHTS, Ivo
Van Roy, Willem
Lagae, Liesbet
Himmelreich, Uwe
Issue Date: 2014
Citation: BIOMATERIALS, 35 (5), p. 1627-1635
Abstract: Magnetic nanoparticle (MNP) enabled cell visualization with magnetic resonance imaging (MRI) is currently an intensively studied area of research. In the present study, we have synthesized polyethylene glycolated (PEG) MNPs and validated their suitability as MR cell labeling agents in in vitro and in vivo experiments. The labeling of therapeutic potent mesenchymal stem cells (MSCs) with small core and large core MNPs was evaluated. Both MNPs were, in combination with a transfection agent, stably internalized into the MSCs and didn’t show an effect on cell metabolism. The labeled cells showed high contrast in MRI phantom studies. For quantification purposes, the MRI contrast generating properties of cells labeled with small core MNPs were compared with large core MNPs and with the commercial contrast agent Endorem. MSCs labeled with the large core MNPs showed the highest contrast generating properties in in vitro phantom studies and in in vivo intracranial stereotactic injection experiments, confirming the sizeerelaxivity relationship in biological systems. Finally, the distribution of MSCs prelabeled with large core PEGylated MNPs was visualized non-invasively with MRI in a glioma model.
Notes: Trekker, J (reprint author), IMEC, Louvain, Belgium. trekker@imec.be
URI: http://hdl.handle.net/1942/16429
DOI: 10.1016/j.biomaterials.2013.11.006
ISI #: 000330156100027
ISSN: 0142-9612
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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