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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/1635

Title: The structural proteome of Pseudomonas aeruginosa bacteriophage phi KMV
Authors: Lavigne, R.
NOBEN, Jean-Paul
Hertveldt, K.
Ceysens, P-J.
Briers, Y.
DUMONT, Debora
Roucourt, B.
Krylov, V.
Mesyanzhinov, Vadim V.
Volckaert, G.
Issue Date: 2006
Publisher: Society for General Microbiology
Citation: MICROBIOLOGY, 152. p. 529-534
Abstract: The structural proteome of {phi}KMV, a lytic bacteriophage infecting Pseudomonas aeruginosa, was analysed using two approaches. In one approach, structural proteins of the phage were fractionated by SDS-PAGE for identification by liquid chromatography-mass spectrometry (LC-MS). In a second approach, a whole-phage shotgun analysis (WSA) was applied. WSA uses trypsin digestion of whole phage particles, followed by reversed-phase HPLC and gas-phase fractionation of the complex peptide mixture prior to MS. The results yield a comprehensive view of structure-related proteins in {phi}KMV and suggest subtle structural differences from phage T7.
URI: http://hdl.handle.net/1942/1635
DOI: 10.1099/mic.0.28431-0
ISI #: 000235272400026
ISSN: 0026-2617
Category: A1
Type: Journal Contribution
Validation: ecoom, 2007
Appears in Collections: Research publications

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