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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16342

Title: Autoantigen induced clonal expansion in immortalized B cells from the peripheral blood of multiple sclerosis patients
Authors: FRAUSSEN, Judith
Vrolix, Kathleen
Martinez-Martinez, Pilar
Losen, Mario
Hupperts, Raymond
Espiño, Mercedes
Villar, Luisa M.
De Baets, Marc H.
SOMERS, Veerle
Issue Date: 2013
Citation: JOURNAL OF NEUROIMMUNOLOGY, 261 (1-2), p. 98-107
Abstract: We studied Ig heavy chain (VDJ) sequences and antigen reactivity of 412 immortalized B cell lines from the peripheral blood of 10 multiple sclerosis (MS) patients, 4 clinically isolated syndrome (CIS) patients and 6 healthy controls (HCs). 78/238 (32.8%) MS and CIS B cell lines were part of 9 clonally expanded B cell populations, of which 5 were present in multiple patients. Increased VH1 gene family usage was evidenced for MS B cells, with 29.2% expressing VH1-69. Affinity maturation in MS and CIS was indicated by increased Ig VDJ mutations. Autoantibody producing B cells reactive to intracellular antigens were significantly higher in MS (25%) and CIS (28%) patients than in HCs (5%), including 3/9 expanded B cell clones. Specificity for phosphatidylcholine was observed for 1/9 B cell clones. These findings indicate clonally expanded autoreactive B cells with affinity maturation in the peripheral blood in MS and CIS
URI: http://hdl.handle.net/1942/16342
DOI: 10.1016/j.jneuroim.2013.05.002
ISI #: 000323459400014
ISSN: 0165-5728
Category: A1
Type: Journal Contribution
Validation: ecoom, 2014
Appears in Collections: Research publications

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