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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/1628

Title: Gel-free analysis of the human brain proteome: Application of liquid chromatography and mass spectrometry on biopsy and autopsy samples
Authors: DUMONT, Debora
NOBEN, Jean-Paul
Issue Date: 2006
Publisher: Wiley
Citation: PROTEOMICS, 60(18). p. 4967-4977
Abstract: This paper reports on the findings of the Biomedical Research Institute, as one of the participants in the pilot study of the HUPO Brain Proteome Project. A biopsy and autopsy study sample derived from human brain was distributed among the participants for proteomic analysis. In our laboratory, attention was focused on protein identification using the bottom-up shotgun approach. Protein extracts derived from both samples were trypsinized and analyzed separately by 2-D LC and MS. In a complementary approach, the tryptic digests were analyzed directly by LC-ESI-MS/MS and gas-phase fractionation in the mass spectrometer. Taken together, both proteomic approaches in combination with a stringent evaluation process, resulted in the confident identification of 209 proteins in the human brain samples under investigation.
URI: http://hdl.handle.net/1942/1628
DOI: 10.1002/pmic.200600080
ISI #: 000240875500008
ISSN: 1615-9853
Category: A1
Type: Journal Contribution
Validation: ecoom, 2007
Appears in Collections: Research publications

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