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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16149

Title: Optimizing the antitumor selectivity of PVP-Hypericin re A549 cancer cells and HLF normal cells through pulsed blue light
Authors: PENJWEINI, Rozhin
Loew, Hans G.
Breit, Paul
Kratkya, Karl W.
Issue Date: 2013
Abstract: Photodynamic therapy (PDT) is based on the preferential accumulation of photosensitizer in cancer cells with subsequent cytotoxicity mediated by singlet oxygen production after light excitation. As photosensitizers accumulate also in the surrounding non-cancer cells, the risk of damaging them by photosensitization is a limitation of PDT. Thus, minimizing the side-effects of PDT on normal cells is one of the challenging problems in medical practice. This paper studies the PDT side-effects of PVP-Hypericin (PVP: polyvinylpyrrolidone) photosensitizer excited with continuous or pulsed irradiation, on combined cell lines of human lung carcinoma epithelial cells (A549) and normal primary human lung fibroblast cells (HLF). In vitro PDTs are performed using pulsed or continuous irradiation with irradiance intensities I* = 1.59, 6.34 and 14.27 mW/cm(2). The LED pulse lengths L are 0.127, 1.29, 13, 54.5 and 131 ms. Then fluorescence and phototoxicity of PVP-Hypericin in the A549 cancer cells are compared with those of HLF normal cells. Although, PVP-Hypericin accumulates more in A549 cancer cells, the results show that HLF cells produce dose-dependent photoreactions in the presence of photosensitizer. PVP-Hypericin induces the most optimized anticancer efficacy with moderate side-effects for I* = 14.27 mW/cm(2) and L = 131 ms. (C) 2013 Elsevier B.V. All rights reserved.
Notes: Penjweini, R (reprint author), Univ Vienna, Fac Phys Phys Physiol Proc, Boltzmanng 5, A-1090 Vienna, Austria. rozhin.penjweini@uhasselt.be
URI: http://hdl.handle.net/1942/16149
DOI: 10.1016/j.pdpdt.2013.06.005
ISI #: 000328666900035
ISSN: 1572-1000
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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