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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/1612

Title: Whole genome phage display selects for proline-rich Boi polypeptides against Bem1p
Authors: Hertveldt, K.
ROBBEN, Johan
Volckaert, G.
Issue Date: 2006
Publisher: Springer
Citation: BIOTECHNOLOGY LETTERS, 28(16). p. 1233-1239
Abstract: Interaction selection by biopanning from a fragmented yeast proteome displayed on filamentous phage particles was successful in identifying proline-rich fragments of Boi1p and Boi2p. These proteins bind to the second ``src homology region 3'' (SH3) domain of Bem1p, a protein of Saccharomyces cerevisiae involved in bud formation. Target Bem1p was a doubly-tagged recombinant, Bem1[Asn142-Ile551], which strongly interacts in ELISA with a C-terminal 75 amino acids polypeptide from Cdc24p exposed on phage. The whole yeast genomic display library contained ~7.7 × 107 independent clones of sheared S. cerevisiae genomic DNA fused to a truncated M13 gene III. This study corroborates the value of fragmented-proteome display to identify strong and direct interacting protein modules.
URI: http://hdl.handle.net/1942/1612
DOI: 10.1007/s10529-006-9082-y
ISI #: 000239085600003
ISSN: 0141-5492
Category: A1
Type: Journal Contribution
Validation: ecoom, 2007
Appears in Collections: Research publications

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