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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16116

Title: Protein Engineering For Directed Immobilization
Authors: STEEN REDEKER, Erik
TA, Duy Tien
BILLEN, Brecht
Issue Date: 2013
Citation: BIOCONJUGATE CHEMISTRY, 24 (11), p. 1761-1777
Abstract: Much effort has been put into the optimization of the functional activity of proteins. For biosensors this protein functional optimization will increase the biosensor's sensitivity and/or selectivity. However, the strategy chosen for the immobilization of the proteins to the sensor surface might be equally important for the development of sensor surfaces that are optimally biologically active. Several studies published in recent years show that the oriented immobilization of the bioactive molecules improves the sensor's properties. In this review, we discuss the state of the art of the different protein immobilization strategies that are commonly used today with a special focus on biosensor applications. These strategies include nonspecific immobilization techniques either by physical adsorption, by covalent coupling, or by specific immobilization via site-specifically introduced tags or bio-orthogonal chemistry. The different tags and bio-orthogonal chemistry available and the techniques to site-specifically introduce these groups in proteins are also discussed.
Notes: Redeker, ES (reprint author), Hasselt Univ, Inst Mat Res IMO, Biomol Design Grp, Div Chem, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium, erik.steenredeker@uhasselt.be
URI: http://hdl.handle.net/1942/16116
DOI: 10.1021/bc4002823
ISI #: 000327413100001
ISSN: 1043-1802
Category: A1
Type: Journal Contribution
Validation: ecoom, 2014
Appears in Collections: Research publications

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