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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16095

Title: reMelting Curve Analysis as a Tool for Enrichment Monitoring in the SELEX Process.
Authors: VANBRABANT, Jeroen
LEIRS, Karen
Issue Date: 2014
Citation: ANALYST, 139 (3), p. 589-595
Abstract: Current aptamer selection procedures enable limited control and transparency on how the DNA selection pool is evolving. Affinity tests and binding analysis are not always as informative. Here we show that real-time PCR provides a valuable tool for the follow-up of aptamer selection. Limited time, work and amount of amplified ssDNA make this an interesting instrument to set-up a SELEX design and monitor the enrichment of oligonucleotides. reMelting Curve Analysis (rMCA) after reannealing at stringent conditions provides information about enrichment, compared to a random library. Monitoring the SELEX process and optimizing conditions by means of the proposed methods can increase the selection efficiency in a controlled way. rMCA is applied in enrichment simulations and three different selection procedures. Our results imply that rMCA can be used for different SELEX designs and different targets. SELEX pool diversity analysis by rMCA is proven to be a useful, reproducible tool to detect and evaluate enrichment of specific binding aptamers while the selection procedure is performed.
Notes: Vanbrabant, J (reprint author),Univ Hasselt, BIOMED, B-3590 Diepenbeek, Belgium, 1jeroenvanbrabant@gmail.com; luc.michiels@uhasselt.be
URI: http://hdl.handle.net/1942/16095
Link to publication: http://pubs.rsc.org/en/content/articlelanding/2013/an/c3an01884a#!divAbstract
DOI: 10.1039/C3AN01884A
ISI #: 000328912000009
ISSN: 0003-2654
Category: A1
Type: Journal Contribution
Validation: ecoom, 2015
Appears in Collections: Research publications

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