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|Title: ||Measuring the intravitreal mobility of nanomedicines with single-particle tracking microscopy|
|Authors: ||Martens, Thomas F.|
Engbersen, Johan F. J.
De Smedt, Stefaan C.
|Issue Date: ||2013|
|Citation: ||NANOMEDICINE, 8 (12), p. 1955-1968|
|Abstract: ||Aim: To develop a robust assay to evaluate and compare the intravitreal mobility of nanoparticles in the intact vitreous body. Materials & methods: Excised bovine eyes were prepared to preserve the fragile structure of the vitreous humor, while permitting high-resolution fluorescence microscopy and single-particle tracking analysis of intravitreally injected nanoparticles. This assay was validated by analyzing polystyrene beads and further employed to evaluate gene nanomedicines composed of poly(amido amine)s and plasmid DNA. Results: The assay was able to distinguish immobilized cationic nanoparticles from mobile PEGylated nanoparticles. PEGylation of the polyplexes resulted in a drastic improvement of their mobility. Conclusion: An ex vivo eye model is presented for studying nanoparticle mobility in intact vitreous humor by single-particle tracking microscopy. These results give important guidelines for developing gene- and drug-delivery nanomedicines that are compatible with intravitreal administration. Original submitted 20 April 2012; Revised submitted 22 November 2012|
|Notes: ||De Smedt, SC (reprint author), De Smedt, SC (reprint author)
De Smedt, SC (reprint author)|
|ISI #: ||000327379600009|
|Type: ||Journal Contribution|
|Validation: ||ecoom, 2014|
|Appears in Collections: ||Research publications|
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