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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/16064

Title: Erwinia amylovora affects the phenylpropanoid-flavonoid pathway in mature leaves of Pyrus communis cv. Conference
Authors: VRANCKEN, Kristof
HOLTAPPELS, Michelle
Schoofs, Johan
Deckers, T.
Treutter, D.
VALCKE, Roland
Issue Date: 2013
Citation: PLANT PHYSIOLOGY AND BIOCHEMISTRY, 72, p. 134-144
Abstract: Flavonoids, which are synthesized by the phenylpropanoid-flavonoid pathway, not only contribute to fruit colour and photoprotection, they also may provide antimicrobial and structural components during interaction with micro-organisms. A possible response of this pathway was assessed in both mature and immature leaves of shoots of 2-year-old pear trees cv. Conference, which were inoculated with the gram-negative bacterium Erwinia amylovora strain SGB 225/12, were mock-inoculated or were left untreated. The phenylpropanoid flavonoid pathway was analysed by histological studies, by gene expression using RT-qPCR and by HPLC analyses of the metabolites at different time intervals after infection. Transcription patterns of two key genes anthocyanidin reductase (ANR) and chalcone synthase (CHS) related to the phenylpropanoid flavonoid pathway showed differences between control, mock-inoculated and E. amy/ovora-inoculated mature leaves, with the strongest reaction 48 h after inoculation. The impact of E. amylovora was also visualised in histological sections, and confirmed by HPLC, as epicatechin which is produced via ANR augmented 72 h after inoculation in infected leaf tissue. Besides the effect of treatments, ontogenesis-related differences were found as well. The increase of certain key genes, the rise in epicatechin and the visualisation in several histological sections in this study suggest a non-negligible impact on the phenylpropanoid flavonoid pathway in Pyrus communis due to inoculation with E. amylovora. In this study, we propose a potential role of this pathway in defence mechanisms, providing a detailed analysis of the response of this system attributable to inoculation with E. amylovora. (C) 2013 Elsevier Masson SAS. All rights reserved.
Notes: Valcke, R (reprint author),Hasselt Univ, Fac Sci, Agoralaan Bldg D, B-3590 Diepenbeek, Belgium. roland.valcke@uhasselt.be
URI: http://hdl.handle.net/1942/16064
DOI: 10.1016/j.plaphy.2013.03.010
ISI #: 000327109800014
ISSN: 0981-9428
Category: A1
Type: Journal Contribution
Validation: ecoom, 2014
Appears in Collections: Research publications

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