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Title: Correlation between hormonal homeostasis and morphogenic responses in Arabidopsis thaliana seedlings growing in a Cd/Cu/Zn multi-pollution context
Authors: Sofo, Adriano
Vitti, Antonella
Nuzzaci, Maria
Tataranni, Giuseppe
Scopa, Antonio
Vangronsveld, Jaco
Remans, Tony
Falasca, Giuseppina
Altamura, Maria M.
Degola, Francesca
di Toppi, Luigi Sanita
Issue Date: 2013
Citation: PHYSIOLOGIA PLANTARUM, 149 (4), p. 487-498
Abstract: To date, almost no information is available in roots and shoots of the model plant Arabidopsis thaliana about the hierarchic relationship between metal accumulation, phytohormone levels, and glutathione/phytochelatin content, and how this relation affects root development. For this purpose, specific concentrations of cadmium, copper and zinc, alone or in triple combination, were supplied for 12days to in vitro growing seedlings. The accumulation of these metals was measured in roots and shoots, and a significant competition in metal uptake was observed. Microscopic analyses revealed that root morphology was affected by metal exposure, and that the levels of trans-zeatin riboside, dihydrozeatin riboside, indole-3-acetic acid and the auxin/cytokinin ratio varied accordingly. By contrast, under metal treatments, minor modifications in gibberellic acid and abscisic acid levels occurred. Real-time polymerase chain reaction analysis of some genes involved in auxin and cytokinin synthesis (e.g. AtNIT in roots and AtIPT in shoots) showed on average a metal up-regulated transcription. The production of thiol-peptides was induced by all the metals, alone or in combination, and the expression of the genes involved in thiol-peptide synthesis (AtGSH1, AtGSH2, AtPCS1 and AtPCS2) was not stimulated by the metals, suggesting a full post-transcriptional control. Results show that the Cd/Cu/Zn-induced changes in root morphology are caused by a hormonal unbalance, mainly governed by the auxin/cytokinin ratio.
Notes: Sofo, A (reprint author), Univ Basilicata, Sch Agr Forestry Food & Environm Sci, Viale Ateneo Lucano 10, I-85100 Potenza, Italy. adriano.sofo@unibas.it
URI: http://hdl.handle.net/1942/16039
DOI: 10.1111/ppl.12050
ISI #: 000326943100004
ISSN: 0031-9317
Category: A1
Type: Journal Contribution
Validation: ecoom, 2014
Appears in Collections: Research publications

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