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Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/15777

Title: The role of collapsin response mediator protein 2 in multiple sclerosis-related T cell functions
Authors: Vangrinsven, Anuschka
Advisors: BUSSCHOTS, Katrien
Issue Date: 2013
Publisher: tUL
Abstract: Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system(CNS)in which T cells play an important role. A proteomics study illustrated the differential expression of collapsin response mediator protein 2 (CRMP2) in the animal model of MS. Additionally, literature showed that phosphorylated CRMP2 is present in chronic MS lesions and that a higher expression of CRMP2 in peripheral T cells correlates with a higher migration of these cells into the CNS. We hypothesized that a knockdown of CRMP2 results in an altered migration and differentiation of T cells, which could improve the clinical outcome in MS and in its animal model. Because T cells are difficult to transfect, we needed to optimize the transfection efficiency. This was the highest when activated Jurkat T cells were transfected using TransFectin in serum-reduced conditions. Next, a knockdown of CRMP2 was established 120 and 144 hours after transfection. The T cell properties could not be investigated.
Notes: master in de biomedische wetenschappen-klinische en moleculaire wetenschappen
URI: http://hdl.handle.net/1942/15777
Category: T2
Type: Theses and Dissertations
Appears in Collections: Master theses

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