Document Server@UHasselt >
Education >
School for Life Sciences >
Master theses >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/15774

Title: Evaluation of iron oxide labeling as a tool for long term cell tracking and its effects on human dental pulp stem cell differentiation
Authors: Ratajczak, Jessica
Advisors: STRUYS, Tom
Issue Date: 2013
Publisher: tUL
Abstract: Human dental pulp stem cells (hDPSCs) are considered plausible candidates for cell-based therapies and non-invasive imaging techniques are necessary to evaluate stem cell therapy. Magnetic labeling with superparamagnetic iron oxide (SPIO) particles, allows in vivo visualization using MRI. This study hypothesized that SPIO particles can be used for long-term in vivo monitoring of hDPSCs, without affecting their differentiation potential. SPIO labeling did not interfere with adipogenic or osteogenic differentiation as evidenced by respectively the co-localization of lipid droplets and SPIO-containing endosomes and the capacity of SPIO-labeled hDPSCs to produce extracellular matrix. In vitro MRI showed that both adipogenic and osteogenic induced SPIO-labeled hDPSCs were able to significantly decrease T2* signal intensity after three weeks of in vitro culture. Furthermore, subcutaneously injected labeled adipogenic induced hDPSCs were detected by in vivo MRI. These results show great promi
Notes: master in de biomedische wetenschappen-klinische en moleculaire wetenschappen
URI: http://hdl.handle.net/1942/15774
Category: T2
Type: Theses and Dissertations
Appears in Collections: Master theses

Files in This Item:

Description SizeFormat
N/A2.06 MBAdobe PDF

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.