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Title: Effects of pH on uranium uptake and oxidative stress responses induced in Arabidopsis thaliana
Authors: Saenen, Eline
Horemans, Nele
Vanhoudt, Nathalie
Vandenhove, Hildegarde
Biermans, Geert
Van Hees, May
Wannijn, Jean
Vangronsveld, Jaco
Cuypers, Ann
Issue Date: 2013
Abstract: Uranium (U) causes oxidative stress in Arabidopsis thaliana plants grown at pH 5.5. However, U speciation and its toxicity strongly depend on environmental parameters, for example pH. It is unknown how different U species determine U uptake and translocation within plants and how they might affect the oxidative defense mechanisms of these plants. The present study analyzed U uptake and oxidative stress-related responses in A. thaliana (Columbia ecotype) under contrasted U chemical speciation conditions. The 18-d-old seedlings were exposed for 3 d to 25 µM U in a nutrient solution of which the pH was adjusted to 4.5, 5.5, 6.5, or 7.5. Results indicate that there is a different rate of U uptake and translocation at the different pHs, with high uptake and low translocation at low pH and lower uptake but higher translocation at high pH. After U exposure, an increased glutathione reductase activity and total glutathione concentration were observed in U-exposed roots, pointing toward an important role for glutathione in the root defense system against U either by chelation or by antioxidative defense mechanisms. In leaves, antioxidative defense mechanisms were activated on U exposure, indicated by increased superoxide dismutase and catalase activity. As it seems that U toxicity is influenced by pH, it is important to consider site-specific characteristics when making U risk assessments. Environ Toxicol Chem 2013;32:2125–2133. © 2013 SETAC
URI: http://hdl.handle.net/1942/15496
DOI: 10.1002/etc.2290
ISI #: 000322253800024
ISSN: 0730-7268
Category: A1
Type: Journal Contribution
Validation: ecoom, 2014
Appears in Collections: Research publications

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